1. College of Food and Biological Engineering, Jimei University, Xiamen, Fujian 361021, China;
2. Key Laboratory of Food Microbiology and Enzyme Engineering Technology of Fujian Province, Xiamen, Fujian 361021, China;
3. Research Center of Food Biotechnology of Xiamen City, Xiamen, Fujian 361021, China
Abstract：The key physicochemical properties of the domestic gracilaria agar and five kinds of commercial biochemical agars were investigated and compared, and the preparation technology of the biochemical agar was further studied by using the carboxymethyl modification method to decrease the melting temperature and promote the transparency of gracilaria agar and further optimized to produce biochemical agar. The results revealed that the gracilaria agar showed related higher melting temperature and lower transparency than these biochemical agars, which were the primary distinctive physicochemical properties between the gracilaria agar and these biochemical agars. The optimized preparation parameters were as follows: the amount of chloroacetic acid was 0.15 g/g and the reaction temperature was 60℃. Under these optimal conditions, 70.8% of Na2SO4 and 57% of NaOH can be reduced respectively through the reutilization of the reaction liquid. The physicochemical properties of synthetic carboxymethyl agar produced by the pilot-scale tests were in accordance with those obtained in the small-scale tests. Meanwhile, the synthetic carboxymethyl agar was consistent with the domestic and international quality standards of the biochemical agars. Moreover, the synthetic carboxymethyl agar possessed a better gel strength and transparency than those commercial biochemical agars. In addition, there is no significant difference between the carboxymethyl agar prepared by the pilot-scale tests and the commercial biochemical agars such as Sigma A6686 and 028990 in the microbial growth tests. The raw materials cost of carboxymethyl agar (1 t/d) is ￥120700.
杜建华 李婷 倪辉 姜泽东 肖安风 朱艳冰. 以江蓠琼脂为原料制备生化琼脂[J]. 过程工程学报, 2018, 18(2): 434-440.
Jianhua DU Ting LI Hui NI Zedong JIANG Anfeng XIAO Yanbing ZHU. Preparation of Biochemical Agar Using Gracilaria Agar as Raw Material. Chin. J. Process Eng., 2018, 18(2): 434-440.
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2018, Vol. 18 
