Simultaneous synthesis of 1,3-dihydroxyacetone (DHA) and 2-aminobutyric acid from glycerol and 2-oxobutyric acid was achieved by coupling the reactions of two enzymes, glycerol dehydrogenase and leucine dehydrogenase with in situ regeneration of coenzyme.
MIAO Wei-juan,WANG Ping,ZHANG Song-ping
National Key Laboratory of Biochemical Engingeering, Institute of Process Engineering, Chinese Academy of Sciences
Abstract：Simultaneous synthesis of 1,3-dihydroxyacetone (DHA) and 2-aminobutyric acid from glycerol and 2-oxobutyric acid was achieved by coupling the reactions of two enzymes, glycerol dehydrogenase and leucine dehydrogenase with in situ regeneration of coenzyme. The effects of concentrations of substrates and coenzyme NAD+ on the yields of the products and the total turnover number (TTN) of the multi-enzyme reaction system were examined. The results show that the TTN value could reach 5110 with an initial NAD+ concentration of 0.1 mmol/L. The observed yields of products were 1.4% and 2.6% for glycerol and 2-oxobutyric acid respectively, based on the amount of substrates used. The strong inhibitory effects of DHA and 2-aminobutyric acid on GDH and LDH were regarded as the main causes for such low product yields. Accordingly, an effective removal strategy of products will be necessary for future improvement of the efficiency of the reaction system.
苗维娟;王平;张松平. 包括辅酶原位再生的偶联酶反应联产1,3-二羟基丙酮和2-氨基丁酸[J]. , 2008, 8(1): 102-108.
MIAO Wei-juan;WANG Ping;ZHANG Song-ping. Simultaneous synthesis of 1,3-dihydroxyacetone (DHA) and 2-aminobutyric acid from glycerol and 2-oxobutyric acid was achieved by coupling the reactions of two enzymes, glycerol dehydrogenase and leucine dehydrogenase with in situ regeneration of coenzyme.. , 2008, 8(1): 102-108.
2008, Vol. 8 
