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›› 2003, Vol. 3 ›› Issue (2): 0-0.

• 2 •    

剪切力对南方红豆杉细胞悬浮培养的影响

韩荣斌，施中东，杨文莉，元英进   

  1. 天津大学化工学院制药工程系，天津 300072
  • 出版日期:2003-04-20 发布日期:2003-04-20

Effect of Shear Stress on Suspension Cultures of Taxus chinensis var. mairei

HAN Rong-bin, SHI Zhong-dong, YANG Wen-li, YUAN Ying-jin   

  1. School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China
  • Online:2003-04-20 Published:2003-04-20

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摘要： 考察了流体剪切力对南方红豆杉悬浮细胞线粒体活性、膜透性、培养液pH、酚积累以及氧迸发等的影响. 结果表明，指数后期或稳定前期的南方红豆杉细胞对剪切力更为敏感，细胞生物活性丧失较大，次生代谢物酚的积累更多；此外，剪切力还引起了红豆杉细胞的防御应答反应，如H+内流、酚类物质大量积累以及活性氧迸发.

关键词: 南方红豆杉细胞, 悬浮培养, 剪切力, 细胞防御应答反应

Abstract: Based on the systematical study of continuous resolution of DL-alanine employing the immobilized Aspergillus oryzae cell, the kinetics of the enzymic reaction was determined. Furthermore, the method of Newton-Raphson was applied to the solution of one-dimension-steady-diffusion model of packed bed for continuous resolution of DL-alanine. The calculated results of the model, with the error less than 10%, were in good agreement with those of experiments. It was demonstrated that the model was reasonable and accurate.

Key words: suspension culture, shear stress, defense responses

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