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›› 2003, Vol. 3 ›› Issue (5): 0-0.

• 5 •    

Production of Citric Acid from Apple Pomace Enzymolyzed by Cellulase

SONG Ji-rong(宋纪蓉)，HUANG Jie(黄洁)，XU Kang-zhen(徐抗震)，ZHAO Qiao-yun(赵巧云)   

  1. College of Chemical Engineering, Shaanxi Key Laboratory of Physical–Inorganic Chemistry,Northwest University, Xi’an, Shaanxi 710069, China
  • 出版日期:2003-10-20 发布日期:2003-10-20

Production of Citric Acid from Apple Pomace Enzymolyzed by Cellulase

SONG Ji-rong,HUANG Jie,XU Kang-zhen,ZHAO Qiao-yun   

  1. College of Chemical Engineering, Shaanxi Key Laboratory of Physical–Inorganic Chemistry,Northwest University, Xi’an, Shaanxi 710069, China
  • Online:2003-10-20 Published:2003-10-20

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摘要： Cellulase can evidently increase the content of glucose and has a significant effect on the production of citric acid from apple pomace by Aspergillus niger. Based on experiments, a cellulolytic enzyme named cellulase A6 was found able to produce about 170 g glucose from 1 kg dried apple pomace after 12 h reaction, with cellulase concentration of 20 U/g in the medium at 50℃, natural pH without pretreatment of alkali. Using the treated apple pomace as a liquid state substrate, Aspergillus niger-C selected out was able to produce about 256 g citric acid from 1 kg dried apple pomace at 35℃ in 3 d or 30℃ in 5 d with flask rotation speed of 210 r/min, and the conversion of citric acid could reach 80% based on the amount of sugar consumed.

关键词: citric acid, apple pomace, cellulase, fermentation

Abstract: Cellulase can evidently increase the content of glucose and has a significant effect on the production of citric acid from apple pomace by Aspergillus niger. Based on experiments, a cellulolytic enzyme named cellulase A6 was found able to produce about 170 g glucose from 1 kg dried apple pomace after 12 h reaction, with cellulase concentration of 20 U/g in the medium at 50oC, natural pH without pretreatment of alkali. Using the treated apple pomace as a liquid state substrate, Aspergillus niger-C selected out was able to produce about 256 g citric acid from 1 kg dried apple pomace at 35oC in 3 d or 30oC in 5 d with flask rotation speed of 210 r/min, and the conversion of citric acid could reach 80% based on the amount of sugar consumed.

Key words: citric acid, apple pomace, cellulase, fermentation

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