Microwave-assisted Aqueous Two-phase Extraction of Steroidal Saponins in Dioscorea zingiberensis
LIU Lin DONG Yue-sheng XIU Zhi-long
Department of Bioscience and Biotechnology, School of Environmental and Biological Science and Technology, Dalian University of Technology Department of Bioscience and Biotechnology, School of Environmental and Biological Science and Technology, Dalian University of Technology Department of Bioscience and Biotechnology, School of Environmental and Biological Science and Technology, Dalian University of Technology
Abstract:Microwave-assisted aqueous two-phase extraction of steroidal saponins in Dioscorea zingiberensis was studied by ethanol/ammonium sulphate system. The results showed that almost all the five main saponins were enriched in the top phase, and the maximum partition coefficients of Zingibernsis newsaponin, deltonin, Diosgenin-triglucoside, Diosgenin-diglucoside and trillin were 89, 91, 93, 117 and 153, respectively. The optimum mass ratio of material to ethanol was 1:21. The optimum system was composed of 25%(w) ethanol and 19%(w) (NH4)2SO4. Under the optimum condition, the yield of total saponins was 95.1%, and the yields of Zingibernsis newsaponin, deltonin, Diosgenin-triglucoside, Diosgenin-diglucoside and trillin were 99.8%, 97.4%, 97.3%, 86.6% and 63.2%, respectively.