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›› 2009, Vol. 9 ›› Issue (6): 1147-1152.

• 生化工程专栏 • 上一篇    下一篇

微波辅助双水相提取盾叶薯蓣中的皂苷成分

刘琳 董悦生 修志龙   

  1. 大连理工大学环境与生命学院生物科学与工程系 大连理工大学环境与生命学院生物科学与工程系 大连理工大学环境与生命学院生物科学与工程系
  • 收稿日期:2009-06-12 修回日期:2009-08-18 出版日期:2009-12-20 发布日期:2009-12-20
  • 通讯作者: 修志龙

Microwave-assisted Aqueous Two-phase Extraction of Steroidal Saponins in Dioscorea zingiberensis

LIU Lin DONG Yue-sheng XIU Zhi-long   

  1. Department of Bioscience and Biotechnology, School of Environmental and Biological Science and Technology, Dalian University of Technology Department of Bioscience and Biotechnology, School of Environmental and Biological Science and Technology, Dalian University of Technology Department of Bioscience and Biotechnology, School of Environmental and Biological Science and Technology, Dalian University of Technology
  • Received:2009-06-12 Revised:2009-08-18 Online:2009-12-20 Published:2009-12-20
  • Contact: XIU Zhi-long

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摘要： 采用微波辅助乙醇/硫酸铵双水相体系，从盾叶薯蓣中提取皂苷成分. 实验结果表明，各种皂苷成分可有效地富集于醇相，盾叶新苷、三角叶皂苷、薯蓣皂苷元-葡萄糖三糖苷、薯蓣皂苷元-葡萄糖二糖苷、延龄草次苷的最大分配系数分别达89, 91, 93, 117和153. 微波辅助双水相提取皂苷成分的最佳药材/乙醇质量比为1:21，最佳相组成为乙醇25%(w)，硫酸铵19%(w)，该条件下的提取率为，总皂苷95.1%，盾叶新苷99.8%，三角叶皂苷97.4%，薯蓣皂苷元-葡萄糖三糖苷97.3%，薯蓣皂苷元-葡萄糖二糖苷86.6%，延龄草次苷63.2%.

关键词: 微波辅助双水相萃取, 乙醇/硫酸铵体系, 盾叶薯蓣, 甾体皂苷, 提取率

Abstract: Microwave-assisted aqueous two-phase extraction of steroidal saponins in Dioscorea zingiberensis was studied by ethanol/ammonium sulphate system. The results showed that almost all the five main saponins were enriched in the top phase, and the maximum partition coefficients of Zingibernsis newsaponin, deltonin, Diosgenin-triglucoside, Diosgenin-diglucoside and trillin were 89, 91, 93, 117 and 153, respectively. The optimum mass ratio of material to ethanol was 1:21. The optimum system was composed of 25%(w) ethanol and 19%(w) (NH4)2SO4. Under the optimum condition, the yield of total saponins was 95.1%, and the yields of Zingibernsis newsaponin, deltonin, Diosgenin-triglucoside, Diosgenin-diglucoside and trillin were 99.8%, 97.4%, 97.3%, 86.6% and 63.2%, respectively.

Key words: microwave-assisted aqueous two-phase extraction, ethanol/ammonium sulphate system, Dioscorea zingiberensis, steroidal saponins, yield