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›› 2003, Vol. 3 ›› Issue (4): 0-0.

• 4 •    

用光谱探针体研究非光谱物质与生物大分子结合作用：十二烷基苯磺酸钠/灿烂甲酚蓝/蛋白质反应体系

沈荣,郑立新,郜洪文   

  1. 1. 安徽大学化学化工学院，安徽 合肥 230039；2. 安徽纺织职业技术学院基础部，安徽 合肥 230051
  • 出版日期:2003-08-20 发布日期:2003-08-20

Preparation of Ultrafine a-Al2O3 Powder by Thermal Decomposition of AACH at Low Temperature

YANG Ye1, WU Yu-cheng1,2, LI Yong2 , CUI Ping2   

  1. 1. Dept. Chem., Anhui Univ., Hefei, Anhui 230039, China; 2. Col. Anhui Textile of Professional Technol., Hefei, Anhui 230051, China
  • Online:2003-08-20 Published:2003-08-20

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摘要： 选择灿烂甲酚蓝(BCB)作为光谱探针体，应用微表面吸附-光谱修正(MSASC)技术，研究了十二烷基苯磺酸钠(SDBS)与3种蛋白质(BSA：牛血清蛋白，OVA：卵清蛋白，Mb：肌红蛋白)的结合反应. 在pH=2.81的酸性介质中，BCB-SDBS和SDBS-蛋白质反应符合Langmuir等温吸附. 通过折点法验证产物的表征数据. 实验表明研究体系中生成了结合物，并考察了温度、盐度对结合溶液的影响，测定了产物结合常数等.

关键词: MSASC技术, 折点方法, 蛋白质组装, Langmuir吸附, 灿烂甲酚蓝, 十二烷基苯磺酸钠

Abstract: The interaction of sodium dodecyl benzene sulfonate (SDBS) with brlliant cresyl blue B (BCB) and three proteins: bovine serum albumin (BSA), myoglobin (Mb) and ovalbumin (OVA) have been investigated based on the theory that the electrostatic attraction between small molecule and biomacromolecule obeys the Langmuir isothermal adsorption. At pH 2.81, the aggregates were characterized by the microsurface adsorption ? spectral correction (MSASC) technique. Results showed that the aggregates: SDBS90×BSA, SDBS41×OVA and SDBS25×Mb at 30°C and SDBS64×BSA, SDBS35×OVA and SDBS15×Mb at 50°C are formed.

Key words: a-Al2O3 ultrafine powder, AACH, preparation, phase transformation

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