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›› 2003, Vol. 3 ›› Issue (5): 0-0.

• 5 •    

固态发酵中纤维素基质降解过程初步研究

周晓宏，陈洪章，李佐虎   

  1. 中国科学院过程工程研究所生化工程国家重点实验室，北京 100080
  • 出版日期:2003-10-20 发布日期:2003-10-20

Experimental Observation on Cellulosic Biodegradation in Solid State Fermentation

ZHOU Xiao-hong, CHEN Hong-zhang, LI Zuo-huo   

  1. Department of Chemical Engineering, Tsinghua University, Beijing 100084, China
  • Online:2003-10-20 Published:2003-10-20

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摘要： 利用斜卧青霉(Penicillium decumbens JUA10)对汽爆麦草和淀粉质的混合基质进行固态发酵，通过分析各种成份及酶活力变化，研究了各成份的降解速率，并探讨了纤维素、半纤维素降解与纤维素酶、半纤维素酶酶活力的关系. 纤维素基质固态发酵中木质纤维素的降解过程实际是同步糖化发酵过程，还原糖不会积累形成对纤维素酶、半纤维素酶的反馈抑制；纤维素降解与纤维素酶的酶活性、半纤维素的降解与半纤维素的酶活性不成正比. 木质纤维素的降解难主要是木质纤维素结构造成的. 半纤维素的降解甚至比纤维素降解更慢，淀粉容易降解，木质素几乎不降解.

关键词: 纤维素, 半纤维素, 纤维素酶, 半纤维素酶, 固态发酵

Abstract: Expression of human Pro-urokinase gene in Pichia pastoris and the influence of main variables on the production and expression of Pichia pastoris were investigated. pH in the growth phase was 5.6, pH in the expression phase was 6.0, and the temperature was always 28oC. It is found that there exists clear corresponding between the AOX and Pro-UK production in the fermentation process, and a new procedure by feeding methanol and glycerol alternatively was proved to be effective in improving the enzyme activity of Pro-UK in the fermentation broth.

Key words: cellulose, hemicellulose, cellulase, hemicellulase, solid state fermentation (SSF)

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