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›› 2003, Vol. 3 ›› Issue (2): 0-0.

• 2 •    

加入有机组份的制备电泳技术分离蛋白质

罗坚,刘铮   

  1. 清华大学化工系,北京 100084
  • 出版日期:2003-04-20 发布日期:2003-04-20

Solvent-containing Preparative Electrophoresis of Proteins

LUO Jian, LIU Zheng   

  1. Xi'an Jiaotong University,Xi'an,Shanxi 710049,China
  • Online:2003-04-20 Published:2003-04-20

摘要: 采用毛细管电泳方法研究了蛋白质在有机-水介质中的电迁移性质,并成功地进行了卵清白蛋白组份I和组份II的分离. 采用荧光分析和圆二色谱法考察了非水组份对蛋白质在溶液中构象的影响,并采用多通道流动电泳设备进行了制备规模的有机-水介质电泳过程研究.

关键词: 有机-水介质, 毛细管电泳, 多通道流动电泳, 电迁移

Abstract: Aimed at directly mine operation conditions, WC particle/grey iron and low-chromium alloy white iron matrix composites were manufactured, and their anti-erosive wear properties were investigated against high-chromium cast iron. Results showed that the erosive wear resistance of composites with different metal matrix materials was better than that of high-Cr cast iron, and that of high-carbon and low-Cr white iron matrix was the best.

Key words: solvent-containing aqueous media, capillary electrophoresis, multichannel flow electrophoresis

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