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›› 2011, Vol. 11 ›› Issue (5): 782-785.

• 反应与分离 • 上一篇    下一篇

水-聚丙二醇两相体系中生物转化法合成2-苯乙醇

陈虹 陈蔚青 张建芬   

  1. 浙江树人大学生物与环境工程学院 浙江树人大学生物与环境工程学院 浙江树人大学生物与环境工程学院
  • 收稿日期:2011-08-01 修回日期:2011-09-30 出版日期:2011-10-20 发布日期:2011-10-20
  • 通讯作者: 陈虹

Synthesis of 2-Phenylethanol by Bioconversion in Aqueous-Organic Solvent Two-phase System

CHEN Hong CHEN Wei-qing ZHANG Jian-fen   

  1. College of Biological and Environmental Engineering, Zhejiang Shuren University College of Biological and Environmental Engineering, Zhejiang Shuren University College of Biological and Environmental Engineering, Zhejiang Shuren University
  • Received:2011-08-01 Revised:2011-09-30 Online:2011-10-20 Published:2011-10-20
  • Contact: CHEN Hong

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摘要： 研究了水-聚丙二醇两相体系中生物转化法合成2-苯乙醇的工艺条件，考察了聚丙二醇分子量、加量、转化振荡转速和底物浓度对2-苯乙醇合成的影响，确定了最佳的转化条件. 结果表明，摇瓶中两相体系转化合成2-苯乙醇的最佳条件为：聚丙二醇分子量1500，加量为培养基体积的1/2，振荡转速250 r/min，底物浓度13 g/L. 在5 L发酵罐中采用水-聚丙二醇两相体系生物转化法合成2-苯乙醇，浓度达6.87 g/L，产率为0.38 g/(L×h)，较常规水相转化分别提高了42.2%和58.3%.

关键词: 2-苯乙醇, 生物转化, 两相体系, 聚丙二醇

Abstract: Production of 2-phenylethanol by bioconversion was performed in a two-phase system composed of water and polypropylene glycol. The molecular weight and adding volume of polypropylene glycol, shaking speed for cultivation and concentration of L-phenylalanine were investigated to improve the productivity of 2-phenylethanol. The bioconversion process was carried out in a 5 L fermentor. The results indicated that the optimal conditions for 2-phenylethanol production in two-phase system were as follows: polypropylene glycol molecular weight was 1500, adding volume half of the volume of medium, shaking speed 250 r/min, and concentration of substrate 13 g/L. In the 5 L fermentor with polypropylene glycol by ISPR, the concentration and productivity of 2-phenylethanol were 6.87 g/L and 0.38 g/(L×h) respectively, compared with no polypropylene glycol extraction, which were improved by 42.2% and 58.3%.

Key words: 2-phenylethanol, bioconversion, two-phase system, polypropylene glycol

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