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›› 2013, Vol. 13 ›› Issue (3): 494-499.

• 生化工程专栏 • 上一篇    下一篇

搅拌槽反应器中中度嗜热浸矿菌预处理含砷金矿

吴俊子 曾伟民 王玉光 仉丽娟 万利利 周洪波   

  1. 中南大学资源加工与生物工程学院 中南大学资源加工与生物工程学院 中南大学资源加工与生物工程学院 中南大学资源加工与生物工程学院 中南大学资源加工与生物工程学院 中南大学资源加工与生物工程学院
  • 收稿日期:2013-04-10 修回日期:2013-05-03 出版日期:2013-06-20 发布日期:2013-06-20
  • 通讯作者: 吴俊子

Pretreatment of Refractory Gold Ore by a Moderate Thermophilic Consortium in Stirred Tank Reactor

WU Jun-zi ZENG Wei-min WANG Yu-guang ZHANG Li-juan WAN Li-li ZHOU Hong-bo   

  1. School of Minerals Processing and Bioengineering, Central South University School of Minerals Processing and Bioengineering, Central South University School of Minerals Processing and Bioengineering, Central South University School of Minerals Processing and Bioengineering, Central South University School of Minerals Processing and Bioengineering, Central South University School of Mineral Processing and Bioengineering, Central South University
  • Received:2013-04-10 Revised:2013-05-03 Online:2013-06-20 Published:2013-06-20
  • Contact: WU Jun-zi

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摘要： 从多个矿坑、煤堆废水中富集中度嗜热浸矿菌，并在搅拌反应器中经过两年的长期驯化，获得能耐受高矿浆浓度和具有抗砷性的浸矿混合菌. 对浸矿混合菌进行耐高矿浆浓度的驯化，保持矿浆浓度为200 g/L，改变两种金矿的配比，提高驯化体系中砷含量，进行耐砷性驯化. 用驯化的浸矿混合菌对难处理金矿进行生物氧化预处理，矿浆浓度为200 g/L，砷含量为8 g/L，14 d时菌体浓度最大，达2′109 mL-1，22 d时砷浸出率为59.93%. 采用限制性酶切片段长度多态性分析对浸矿14和22 d时的微生物多样性进行分析，发现存在Sulfobacillus sp., Acidithiobacillus caldus, Ferroplasma thermophilum和Pseudomonas sp..

关键词: 难处理金矿, 搅拌槽反应器, 中度嗜热浸矿菌, 预处理, 限制性酶切片段长度多态性分析

Abstract: A moderate thermophilic consortium was enriched from acid mine drainage and coal mine wastewater samples collected in China. After adaptation to gradually increased pulp density of arsenic-bearing refractory gold ore and concentration of arsenic by serial sub-culturing for about two years, its tolerance ability was up to 200 and 8 g/L, respectively. After that, the consortium was employed to treat arsenic-bearing refractory gold ore to evaluate its ability and potential for industrial application. The results indicated that the maximum cell density was 2′109 mL-1 (the 14th day) during the whole process and arsenic extraction rate reached up to 59.93% in 22 d. Microbial diversity monitored by restriction fragment length polymorphism suggested that the consortium mainly consisted of four species, Sulfobacillus sp., Acidithiobacillus caldus, Ferroplasma thermophilum and Pseudomonas sp.

Key words: refractory gold ore, stirred tank reactor, moderate thermophile, pretreatment, restriction fragment length polymorphism

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