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›› 2005, Vol. 5 ›› Issue (2): 188-192.

• 生化工程专栏 • 上一篇    下一篇

两株假单胞菌对烷烃的摄取和降解

杨国君,宋若海,华兆哲,李华钟,陈坚   

  1. 江南大学 生物工程学院
  • 出版日期:2005-04-20 发布日期:2005-04-20

Uptaking and Biodegradation of Alkane by Two Pseudomonas Strains

YANG Guo-jun,SONG Ruo-hai,HUA Zhao-zhe,LI Hua-zhong,CHEN Jian   

  1. School of Biotechnology, Southern Yangtze University
  • Online:2005-04-20 Published:2005-04-20

摘要: 根据细菌在油水混合体系中的分布差异,从江苏油田附近污染的土样中筛选得到了两株对烷烃具有降解能力的假单胞菌Pseudomonas sp. A和Pseudomonas sp. B. 在以煤油为唯一碳源的培养基中,Pseudomonas sp. A的生长量较低,菌体主要聚集在油相中,菌体细胞呈亲油性,生长几乎不受生物表面活性剂抑制剂EDTA的影响,菌体的生长对培养基的表面张力的降低幅度较小;而Pseudomonas sp. B则表现相反的性质. 说明两菌株对烷烃具有不同的摄取机制,Pseudomonas sp. A是以直接接触烷烃的方式摄取,而Pseudomonas sp. B则是通过产生生物表面活性剂对烷烃进行乳化后再摄取.

关键词: 假单胞菌, 烷烃, 摄取, 生物表面活性剂

Abstract: Pseudomonas sp. A and Pseudomonas sp. B, which could biodegrade alkane, were isolated from petroleum-contaminated soil nearby Jiangsu Oil Field through bacterial distribution in oil-water two phases system. When Pseudomonas sp. A was incubated in the medium with kerosene as sole carbon source, its growth was slow, the bacteria mainly congregated in oil phase and showed high microbial adhesion to hydrocarbons, the growth was not affected by EDTA, the inhibitor of the biosurfactant production, the surface tension of incubation broth decreased only slightly; as to Pseudomonas sp. B, it showed the opposite features. The above results indicated the different mechanisms of alkane uptake in two Pseudomonas strains, Pseudomonas sp. A uptakes kerosene by the direct contact mechanism, while Pseudomonas sp. B uptakes the kerosene emulsified with the biosurfactant produced by the cells.

Key words: Pseudomonas, alkane, uptake, biosurfactant