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›› 2009, Vol. 9 ›› Issue (2): 355-359.

• 生化工程专栏 • 上一篇    下一篇

表面活性剂对麦草同步糖化发酵转化乙醇的影响

罗鹏 刘忠   

  1. 天津商业大学机械工程学院 天津科技大学材料科学与化学工程学院
  • 收稿日期:2008-08-28 修回日期:2009-01-12 出版日期:2009-04-20 发布日期:2009-04-20
  • 通讯作者: 罗鹏

Effects of Surfactants on Simultaneous Saccharification and Fermentation of Wheat Straw to Ethanol

LUO Peng LIU Zhong   

  1. College of Mechanical Engineering, Tianjin University of Commerce College of Material Science and Chemical Engineering, Tianjin University of Science and Technology
  • Received:2008-08-28 Revised:2009-01-12 Online:2009-04-20 Published:2009-04-20
  • Contact: LUO Peng

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被引次数

30

摘要： 研究了5种非离子型表面活性剂(BSA, Tween-20, Tween-80, PEG-4000, PEG-6000)促进麦草同步糖化发酵的效果. 结果表明，5种表面活性剂均能促进麦草同步糖化发酵，以Tween-20效果最为显著. 反应体系中添加Tween-20可降低酶用量而保持乙醇浓度基本相同. 在pH 5.0、温度37℃、底物浓度50 g/L及Celluclast 1.5 l用量25 FPU/g、Novozym 188用量15 IU/g的反应体系中，添加0.03 g/g Tween-20，反应72 h，乙醇浓度达到18.7 g/L，比未添加表面活性剂的体系提高了14.0%，反应时间缩短了12 h.

关键词: 麦草, 表面活性剂, 同步糖化发酵, 乙醇

Abstract: Five different non-ionic surfactants, BSA, Tween-20, Tween-80, PEG-4000 and PEG-6000, were evaluated for their ability to enhance simultaneous saccharification and fermentation (SSF) of wheat straw. The results indicated that all non-ionic surfactants tested improved the SSF of wheat straw, and Tween-20 was the most effective. Addition of Tween-20 decreased the enzyme loading while retaining the same ethanol concentration. The ethanol concentration of 18.7 g/L was achieved at a substrate concentration of 50 g/L at 37℃ and pH 5.0 using cellulase mixture (25 FPU/g of substrate for Celluclast 1.5 l and 15 IU/g for Novozym 188) for 72 h. Compared with the reference run without Tween-20, the ethanol concentration was increased by 14.0% whereas the residence time reduced by 12 h.

Key words: wheat straw, surfactant, simultaneous saccharification and fermentation, ethanol

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