欢迎访问过程工程学报, 今天是

›› 2003, Vol. 3 ›› Issue (5): 0-0.

• 5 •    

产碱性脂肪酶菌株的筛选和培养

洪骏,夏黎明   

  1. 浙江大学材料与化工学院化学工程与生物工程系, 浙江 杭州 310027
  • 出版日期:2003-10-20 发布日期:2003-10-20

Self-assembly Mechanism and Defect Analysis of Colloidal Silica Photonic Crystals

ZHAO Xiao-feng1,ZHANG Hui1,2,TANG Qing1   

  1. 1. Inst. Proc. Eng., CAS, Beijing 100080, China; 2. Univ. Sci. & Technol. Beijing, Beijing 100083, China
  • Online:2003-10-20 Published:2003-10-20

PDF

摘要： 以Rodamine B平板筛选法获得了一株高活力碱性脂肪酶产生菌Pseudomonas sp. ZJU-02.摇瓶产酶实验表明，适宜产酶培养基为(%)：玉米浆3，植物油1, K2HPO4 0.1, KCl 0.05, MgSO4 0.05, Tween80 0.05；最佳培养条件为温度26℃、初始pH 6.5. 脂肪酶活力可高达86 IU/ml. 该酶在70℃以下、pH 7.0~10.5范围内稳定；酶反应的适宜温度为40℃，适宜pH为9.5. 该酶制剂在洗涤剂和制革工业中有良好的应用前景.

关键词: 碱性脂肪酶, 菌种筛选, 酶学特性

Abstract: Colloidal silica photonic crystals were prepared by using ethanol as medium. Nanospheres with a mean diameter of 238 nm and standard deviation 5.7% were obtained under strictly controlled conditions by using the improved Stober method. In this method, ethanol was proved a better medium than water and the prepared photonic crystals had fewer defects. The diffraction to incident infrared light obeyed the Bragg equation. The formation mechanism and defect formation of colloidal photonic crystals were also discussed.

Key words: photonic crystal, monodispersed, silica, growth mechanism, defect

中图分类号: