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›› 2008, Vol. 8 ›› Issue (2): 333-338.

• 生化工程专栏 • 上一篇    下一篇

产朊假丝酵母发酵生产谷胱甘肽的氨基酸添加策略

尹良鸿 吴晓玉 廖鲜艳 堵国成 陈坚   

  1. 江南大学 江南大学教育部工业生物技术重点实验室 江南大学生物工程学院环境生物技术研究室 江南大学工业生物技术教育部重点实验室 江南大学工业生物技术教育部重点实验室
  • 收稿日期:2007-10-16 修回日期:2008-01-08 出版日期:2008-04-20 发布日期:2008-04-20
  • 通讯作者: 堵国成

Cultivation of Candida utilis WSH02-08 for Production of Glutathione by Amino Acids Addition Strategy

WU Xiao-yu DU Guo-cheng CHEN Jian   

  1. Key Laboratory of Industrial Biotechnology, Ministry of Education Lab of Environmental Biotechnology, School of biotechnology Southern Yangtze University Key Lab. Industrial Biotechnol., Ministry of Education, Jiangnan University
  • Received:2007-10-16 Revised:2008-01-08 Online:2008-04-20 Published:2008-04-20
  • Contact: DU Guo-cheng

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被引次数

24

摘要： 研究了添加氨基酸对产朊假丝酵母(Candida utilis WSH02-08)合成谷胱甘肽(Glutathione, GSH)的影响，结果表明，两阶段添加氨基酸能显著提高GSH的发酵水平. 摇瓶中发酵10 h添加4 mmol/L L-半胱氨酸，16 h添加混合氨基酸(L-谷氨酸8 mmo/L，L-半胱氨酸8 mmol/L，甘氨酸12 mmol/L)，GSH产量和胞内GSH含量分别为290.1 mg/L和3.22%. 在3 L发酵罐中，将上述氨基酸添加策略与高密度培养技术相结合，36 h流加葡萄糖的同时添加20 mmol/L L-胱氨酸，45 h添加混合氨基酸(L-谷氨酸40 mmo/L，L-半胱氨酸40 mmol/L，甘氨酸60 mmol/L)，GSH产量和胞内GSH含量分别达1725.3 mg/L和3.29%，分别比高密度培养提高了111.4%和100.6%.

关键词: 谷胱甘肽, 产朊假丝酵母, 高密度培养, 氨基酸添加策略

Abstract: The effects of amino acids addition on glutathione production of C. utilis WSH 02-08 were investigated. The results showed that two-step amino acids addition could greatly increase GSH production level. The optimal conditions for two-step amino acids addition strategy were obtained as follows: 4 mmol/L L-cysteine was added after 10 h cultivation, and a mixture of three amino acids (L-glutamic acid, L-cysteine and glycine) was added at 16 h. The optimum concentrations of those three amino acids (8 mmol/L L-glutamic acid, 8 mmol/L L-cysteine and 12 mmol/L glycine) were obtained by orthogonal experiments, GSH concentration and intracellular GSH content reached to 290.1 mg/L and 3.22%. Combining the amino acids addition strategy and high-cell-density cultivation by glucose feeding, 20 mmol/L L-cysteine was added at 36 h, and then the three amino acids (40 mmol/L L-glutamic acid, 40 mmol/L L-cysteine and 60 mmol/L glycine) were added at 45 h, GSH concentration and intracellular GSH content reached to 1725.3 mg/L and 3.29%, which were higher (111.4% and 100.6%) than those of high-cell-density cultivation (816 mg/L and 1.64%).

Key words: glutathione, Candida utilis, high-cell-density cultivation, amino acids addition strategy

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