Abstract: The metabolic characteristics under four operation ways of batch fermentation, constant feeding rate fermentation, changing feeding rate fermentation and batch feeding fermentation, were studied in a 5 L fermentator. And the influences of different S-adenosyl-L-methionine (SAM) fermentation modes were investigated. The optimum fermentation mode among them of Saccharomyces cerevisiae HYS98 was batch feeding. The average productivity and cell growth rate of the feed batch fermentation were obtained as 0.123 g/(L×h) and 1.030 g/(L×h), which were 68% and 73% higher than the highest levels reported in the literature respectively. The results laid the foundations for further optimization of SAM fermentation process.

Key words: Saccharomyces cerevisiae HYS98, S-adenosyl-L-methionine, fermentation mode

摘要： 分别研究了分批发酵、恒速流加发酵、变速流加发酵和分批补料发酵4种操作方式下酿酒酵母HYS98发酵生产SAM的代谢特征，并考察了不同发酵方式对发酵结果的影响. 结果表明，酿酒酵母HYS98发酵生产SAM的最佳发酵模式为分批补料发酵，SAM的平均产率和菌体生长速率分别达0.123和1.030 g/(L×h)，比国内外文献报道的最高水平分别提高了68%和73%，为发酵工艺进一步优化奠定了基础.

关键词: 酿酒酵母HYS98, S-腺苷甲硫氨酸, 发酵方式