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›› 2009, Vol. 9 ›› Issue (3): 456-461.

• 反应与分离 • Previous Articles     Next Articles

Preparation of Pluronic-based Magnetic Nanoparticles and Their Application in Purification of Laccase

LIU Xing-hua GUO Chen YANG Liang-rong WANG Feng LIU Chun-zhao LIU Hui-zhou   

  1. Key Lab. Green Process & Eng., Institute of Process Engineering, Chinese Academy of Sciences Young Scientist Laboratory of Separation Science and Engineering, State Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences Young Scientist Laboratory of Separation Science and Engineering, State Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences Young Scientist Laboratory of Separation Science and Engineering, State Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences State Key Laboratory of Biochemical Engineering, Institute of Process Engineering Institute of Process Engineering, Chinese Academy of Sciences
  • Received:2009-01-16 Revised:2009-02-25 Online:2009-06-20 Published:2009-06-20
  • Contact: LIU Xing-hua

Pluronic嵌段共聚物磁性纳米颗粒的制备及其对漆酶的分离纯化

刘兴华 郭晨 杨良嵘 王锋 刘春朝 刘会洲   

  1. 中国科学院过程工程研究所绿色过程与工程重点实验室 中科院过程工程研究所分离科学与工程青年实验室、生化工程国家重点实验室、中科院研究生院 中科院过程工程研究所分离科学与工程青年实验室、生化工程国家重点实验室、中科院研究生院 中科院过程工程研究所分离科学与工程青年实验室、生化工程国家重点实验室、中科院研究生院 中国科学院过程工程研究所生化工程国家重点实验室 中国科学院过程工程研究所分离工程与工程青年实验室
  • 通讯作者: 刘兴华

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Abstract: Nano-sized monodispersed Pluronic (PEO-PPO-PEO)-based magnetic nanoparticles (PMNPs) were synthesized as carriers for the purification of laccase from the mycelium culture of Trametes versicolor. The adsorption assay was carried out in model system of laccase. The result showed that the maximum adsorption capacity was 0.535 mg/mg without any decrease in the range from 5℃ to 40℃. Purification of laccase from mycelium culture by PMNPs was also investigated. Total enzyme activity recovery rate could reach 62.9% with a single step, and purification fold 3.4.

Key words: PEO-PPO-PEO, magnetic nanoparticles, adsorption, purification, laccase

摘要： 选择合适的双亲性嵌段共聚物Pluronic产品接枝到Fe3O4粒子的表面，合成了粒径均一、亲水性好、非特异性吸附低的纳米级磁性分离载体(PMNPs). 通过对漆酶发酵液的分离纯化研究了Pluronic对排除蛋白非特异性吸附的作用. 结果表明，PMNPs对漆酶的最大吸附量可达0.535 mg/mg，在5~40℃内基本不受温度变化影响，对漆酶发酵液的分离纯化因子为3.4，酶活收率达62.9%，可实现一步分离提纯.

关键词: PEO-PPO-PEO, 磁纳米颗粒, 吸附, 分离纯化, 漆酶

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