Abstract: There is product inhibition in the bioconversion from isoeugenol to vanillin by crude enzyme extracted from soybean. The product inhibition can be prevented effectively with the addition of resin HD-8. The optimum concentration of substrate is 1.5% (φ). With 150 g/L HD-8 added at the beginning, the vanillin concentration reached its maximum (3.41 g/L), corresponding to a molar conversion rate of 24.53%. As a result, the substrate and product can be separated efficiently. The yield of product and the recovery of substrate are 67.11% and 71.88% respectively.

Key words: enzymatic conversion, vanillin, isoeugenol, reaction integrated with separation

摘要： 大豆脂氧合酶粗酶转化异丁香酚制备香草醛的过程中存在产物抑制酶转化现象，吸附剂HD-8树脂可以有效解除产物抑制，提高转化率. 确定了加入底物量1.5%(φ)、树脂量150 g/L的优化转化工艺，使反应与分离进行很好的耦合，既提高了转化率，又能较好地实现底物和产物的分离. 在优化条件下香草醛最高转化量可达3.41 g/L，相应的摩尔转化率为24.53%. 树脂洗脱法提取的产物得率为67.11%，底物的回收率为71.88%.

关键词: 酶法转化, 香草醛, 异丁香酚, 反应分离耦合