Abstract: Glycylglycine imprinted polymer microspheres (Gly-Gly-MIPMs) were synthesized by emulsion polymerization using glycylglycine as template, acrylamide as monomer and ethylene glycol dimethacrylate as crosslinker. The preparation conditions on the water phase morphology and size distributions of the influence and the polymer microspheres adsorption properties were discussed. The optimum preparation conditions were: mass ratio of emulsifier Tween-80 to Span-80 3:1, amount of 5% of monomer, initiator dosage of 1.1% monomer, reaction temperature 45℃, and stirring speed 450 r/min. Gly-Gly-MIPMs were monodisperse, with an average diameter of about 100 nm, imprinted polymers on two-Gly peptides showed better specific adsorption, which contained two different kinds of binding sites, and their imprinting factor was up to 2.18.

Key words: glycylglycine, molecular imprinting, polymeric microspheres, emulsion polymerization

摘要： 以双甘氨肽(Gly-Gly)为模板分子、丙烯酰胺为单体、乙二醇二甲基丙烯酸酯为交联剂，采用乳液聚合法制备了双甘氨肽分子印迹聚合物微球(Gly-Gly-MIPMs)，讨论了水相制备条件对其平均粒径及粒径分布的影响及聚合物微球的吸附特性. 较优的制备工艺条件为：乳化剂Tween-80与Span-80质量比3:1，用量为单体用量的5%，引发剂用量为单体用量的1.1%，反应温度45℃，搅拌速率450 r/min. 合成的Gly-Gly-MIPMs呈单分散性，平均粒径约100 nm；印迹聚合物对双甘氨肽分子表现出较好的特异性吸附，其含有2类不同的结合位点，印迹因子高达2.18.

关键词: 双甘氨肽, 分子印迹, 聚合物微球, 乳液聚合