Abstract: Enzymatic synthesis of triglyceride directly from glycerol with EPA or DHA was studied by the lipase, respectively. The reaction medium, temperature, amount of lipase, molar ratio of glycerol to EPA, addition time of molecular sieves, and reaction time were examined predominantly. The optimal reaction mixture and conditions were: 6 mL hexane, molar ratio of glycerol/EPA or glycerol/DHA 1:3 (based on 0.4 mmol glycerol), 100 mg of Novozym 435 lipase, 40℃, agitation speed 150 r/min, and 1 g molecular sieve added after 24 h reaction time. After 48 h of reaction under these conditions, the yields of tri-EPA and tri-DHA were up to 88.64% and 88.07%, respectively. Meanwhile the degrees of EPA and DHA esterification were 95.0% and 94.5%. The products were identified as tri-EPA and tri-DHA.

Key words: tri-EPA/DHA, lipase, lipase-catalyzed synthesis

摘要： 利用脂肪酶分别催化游离型EPA和DHA与甘油发生酯化反应生成甘油三酯，考察了合成的影响因素. 结果表明，正己烷6 mL，甘油/EPA(DHA)摩尔比为1:3(以0.4 mmol甘油为基准)，Novozym 435添加量为100 mg，反应温度40℃，振荡频率150 r/min，反应24 h后添加1 g分子筛，反应时间48 h，EPA甘油三酯与DHA甘油三酯的得率分别可达88.64%和88.07%，EPA与DHA酯化度分别可达95.0%和94.5%. 分析结果表明，所得产物为EPA甘油三酯和DHA甘油三酯.

关键词: EPA/DHA甘油三酯, 脂肪酶, 酶法合成