Abstract: Animal model of type 2 diabetes mellitus was induced by high-glucose and high-fat diet combined with low-dose streptozotocin for partial destruction of pancreatic b cells. The rats in model group were intraperitoneally injected with streptozotocin (30 mg/kg) after feeding with high-glucose and high-fat diet for 4 weeks. The value of fasting blood glucose greater than 13.8 mmol/L was 86.7% after the injection for one week, and remained stable in 8 weeks. Glucose tolerance test showed that glucose tolerance was impaired in the model group. The hypoglycemic effect of model group was significant after subcutaneous injection of Exenatide (50 mg/kg), after 3 h the glucose level fell by 38.2% compared with the initial level, showing model rats responded well to Exenatide. The rats were sacrificed to harvest the pancreas for pathological examination in the 13th week, the results showed that part of the islet b cells of model rats was destroyed. The method could be used for pharmacodynamic study in related research.

Key words: type 2 diabetes mellitus, streptozotocin, animal model, Exenatide, evaluation

摘要： 通过高糖高脂饮食诱导，联合小剂量链脲佐菌素部分破坏胰岛b细胞，建立II型糖尿病SD大鼠模型. 结果表明，模型组大鼠饲喂高糖高脂饲料4周后，腹腔注射30 mg/kg链脲佐菌素，1周后86.7%的大鼠空腹血糖高于13.8 mmol/L，在8周观察期内稳定，糖耐量受损. 皮下注射50 mg/kg艾塞那肽降血糖作用明显，3 h时较初始水平下降38.2%，表明糖尿病大鼠对II型糖尿病治疗药物艾塞那肽的反应良好. 第13周处死大鼠，胰脏病理切片检查，模型组大鼠部分胰岛b细胞被破坏. 建立的模型可用于药效学的相关评价研究.

关键词: II型糖尿病, 链脲佐菌素, 动物模型, 艾塞那肽, 评价