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›› 2010, Vol. 10 ›› Issue (5): 899-904.

• 反应与分离 • Previous Articles     Next Articles

Desalination of Immune Activity Peptide Extracted from Pollock Frame with Macroporous Polystyrene Resins

HOU Hu ZHAO Xue ZHANG Zhao-hui LI Ping-lin LI Ba-fang   

  1. College of Food Science and Engineering, Ocean University of China, Shandong Province College of Food Science and Engineering, Ocean University of China, Shandong Province College of Food Science and Engineering, Ocean University of China, Shandong Province College of Food Science and Engineering, Ocean University of China, Shandong Province
  • Received:2010-07-09 Revised:2010-09-07 Online:2010-10-20 Published:2010-10-20
  • Contact: HOU Hu

大孔聚苯乙烯吸附树脂对鳕鱼排免疫活性肽的脱盐

侯虎 赵雪 张朝辉 李平林 李八方   

  1. 中国海洋大学食品科学与工程学院 中国海洋大学食品科学与工程学院 中国海洋大学食品科学与工程学院 中国海洋大学食品科学与工程学院
  • 通讯作者: 侯虎

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Abstract: To study an economical and efficient desalting method of immune activity peptide from pollock frame, the six macroporous polystyrene resins (DA201, DA-201B, DA201-C, D3520, D4006 and AB-8) were used in this work. The effects of different influential factors (temperature, pH, concentration, solid-liquid ratio, etc.) on the adsorbing capacity of resins were investigated. In dynamic adsorption and desorption, the desalting rate, peptide recovery rate, and spleen cell proliferation were studied. The results showed that the highest adsorbing rate was 84.7% under 25℃, pH 4.0, the solution 10 mg/mL, and the ratio of resin mass to the volume of peptide 2 g/mL. The desalting and recovery rates of peptides were 98.1% and 90.3%, respectively. The sample desalted by DA201-C resin could enhance the splenocyte proliferation in a dose-dependent manner.

Key words: pollock frame, immune activity peptide, macroporous resin, desalination

摘要： 比较了6种聚苯乙烯型大孔树脂对鳕鱼排活性肽的吸附能力，并研究了各因素(温度、pH、吸附物浓度、料液比等)对树脂吸附特性的影响. 通过动态吸附与解析实验，探讨了DA201-C树脂对鳕鱼排活性肽脱盐的可行性. 结果表明，DA201-C树脂对鳕鱼排活性肽吸附能力最强，在温度25℃、pH 4.0、溶液浓度10 mg/mL、料液比(树脂质量与活性肽体积比)2 g/mL的条件下，吸附率最高为84.7%. 在动态吸附解析实验中，多肽的脱盐率为98.1%，回收率为90.3%. 且脱盐后的多肽同样具有促进脾细胞增殖活性且呈剂量依赖关系.

关键词: 鳕鱼排, 免疫肽, 大孔树脂, 脱盐

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