Abstract: To promote the production of ?-ketoglutaric acid (?-KG) by Yarrowia lipolytica WSH-Z06 with glycerol as the carbon source, the effects of pH on cell growth and ?-KG production were compared, and a strategy of controlling biomass by pH regulation with analysis of kinetic characteristics was established. Furthermore, the effect of biomass concentration on ?-KG production was compared. The results showed that the pH value was maintained at 6.0 at beginning of fermentation, and decreased naturally to 3.0 after the biomass concentration reached up to 10 g/L, then maintained till the end of fermentation. Using this strategy, the maximum ?-KG production reached up to 54.0 g/L, the production and productivity were 19.3% and 17.8% higher than the corresponding values of control group, respectively.

Key words: pH regulation, biomass concentration, specific production rate

摘要： 为了促进解脂亚洛酵母(Yarrowia lipolytica WSH-Z06)利用甘油高效生产?-酮戊二酸，比较了pH 值对菌体生长和 产?-酮戊二酸的影响，通过动力学分析确定了一种通过调控pH 值控制菌体浓度促进产?-酮戊二酸的策略，比较了菌体浓 度对产?-酮戊二酸的影响. 结果表明，发酵初期控制pH 为6.0，菌体浓度达10 g/L 后pH 自然降至3.0 保持到发酵结束， ?-酮戊二酸产量达54.0 g/L，产量和生产强度分别比以CaCO3 为pH 缓冲剂时提高了19.3%和17.8%.

关键词: pH 调控, 菌体浓度, 比生产速率