Abstract: In order to study the effect of hydrolase on the gas production performance of mixed anaerobic digestion of residual sludge and kitchen waste leachate, the residual sludge and kitchen waste leachate from urban sewage treatment plants were used as substrates, and the mixing ratio was 1:1 and 2:1, and protease and cellulase were added in the ratio of 1:1, 1:2, and 2:1 (total enzyme addition amount: 60 mg/g TS) to the mixed solution, and mixed anaerobic digestion at a temperature of (38±0.5)℃. The results showed that the gas production performance and disposal effect was the best when protease and cellulase were added in 1:1 in the 2:1 mixed anaerobic digestion system of residual sludge and kitchen waste leachate. Its daily methane production peak is 60.15 mL/g VS, which is 1.84 times higher than that of sludge digestion alone, and 70.49% higher than that of the same substrate without enzymes; the total methane production was 296.17 mL/g VS, which was 75.99% higher than that of sludge digestion alone, and 62.87% higher than that of the same substrate without enzymes; the removal rates of total solid (TS) and volatile solid (VS) were 41.43% and 67.32%, respectively, which were 14.74 and 27.89 percentage point higher than that of sludge digestion alone, and 8.34 and 21.99 percentage point higher than that of the mixed solution without enzymes in the same group, and there was no ammonia inhibition and VFAs accumulation during the digestion process. Using three-dimensional fluorescence spectroscopy combined with parallel factor analysis, it was found that the fluorescent substances present in the mixed anaerobic digestion process were mainly protein-like, fulvic-like, and humic-like. There were significant differences in protein fluorescence intensity in different time periods of digestion. This change was more pronounced in the enzyme-added experimental group. The results of this study can provide a reference for the reduction and recycling of excess sludge and kitchen waste.

Key words: hydrolysis enzyme, excess sludge, kitchen waste leachate, anaerobic digestion, three-dimensional excitation-emission matrix fluorescence spectroscopy

摘要： 为了研究水解酶对剩余污泥和厨余垃圾渗滤液混合厌氧消化产气性能的影响，以城市污水处理厂剩余污泥和厨余垃圾渗滤液为底物，在混合比为1:1和2:1混合液中分别加入比例为1:1, 1:2和2:1 (总酶加入量60 mg/g TS)的蛋白酶和纤维素酶，在38±0.5℃的温度下进行混合厌氧消化。结果表明，在剩余污泥与厨余垃圾渗滤液2:1混合厌氧消化体系中1:1投加蛋白酶和纤维素酶时产气性能和处置效果最佳。其日产甲烷峰值为60.15 mL/g VS，比单独消化污泥提高了1.84倍，比同底物不加酶时提高了70.49%；甲烷总产量为296.17 mL/g VS，比单独消化污泥提高了75.99%，比同底物不加酶时提高了62.87%；总固体(TS)和挥发性固体(VS)去除率分别为41.43%和67.32%，比单独消化污泥分别提高了14.74和27.89个百分点，比同组不加酶的混合液分别提高了8.34和21.99个百分点，且消化过程中没有出现氨抑制和挥发性脂肪酸(VFAs)积累的状况。利用三维激发矩阵荧光光谱结合平行因子法分析，发现混合厌氧消化过程中存在的荧光物质主要有类蛋白质、类富里酸和类胡敏酸，在消化的不同时间段蛋白质荧光强度存在明显差异，加酶的实验组中这一变化更加显著。此结果可为剩余污泥和厨余垃圾的减量化和资源化提供借鉴。

关键词: 水解酶, 剩余污泥, 厨余垃圾渗滤液, 厌氧消化, 三维激发矩阵荧光光谱