Abstract: To improve the domestication efficiency of Acidithiobacillus ferrooxidans and obtain strains of Acidithiobacillus ferrooxidans which can endure high concentrations of uranium and fluoride ions, the domestication methods of uranium and fluoride resistance were studied, continuous transfer domestication and associated domestication were put forward. The results showed that the continuous transfer domestication could accelerate the domestication speed and increase the domestication effect, and one strain with the Fe2+ oxidation activity of 7.5 times higher than wild strains in the medium containing 1000 mg/L U6+ was obtained in 96 h. Furthermore, the strains which could tolerate 1500 mg/L U6+ or 80 mg/L F- were obtained using this method. As for the associated domestication, it could acquire the strains with stronger oxidation activity in the medium containing both uranium and fluoride than that of tradition methods. The best results from the group carried through uranium domestication before fluoride domestication, and its Fe2+ oxidation activity was at least 6 times higher than those obtained by the other methods in the medium containing 1000 mg/L U6+ and 80 mg/L F-.

Key words: Acidithiobacillus ferrooxidans, domestication, uranium, fluoride

摘要： 为了提高氧化亚铁硫杆菌(Acidithiobacillus ferrooxidans, A.f)的驯化效率，获得能耐受高浓度铀和氟离子的A.f菌株，对分离纯化得到的A.f菌株SKS10的耐铀、耐氟驯化方法进行了研究，提出了连续转接驯化和联合驯化方法. 结果表明，连续转接驯化法可加快驯化速度，提高驯化效果，96 h可获得氧化速率提高7.5倍、能耐受1000 mg/L U6+的菌株，且能获得用传统驯化方法不能得到的耐受1500 mg/L U6+的菌株及能耐受120 mg/L F-的菌株. 与传统驯化法相比，联合驯化法可获得能同时耐受较高铀和氟离子且氧化活性较强的菌株，先进行耐铀驯化再进行耐氟驯化效果更好，在含1000 mg/L U6+和80 mg/L F-的培养基中，Fe2+的氧化速率是其他驯化方法的6倍以上.

关键词: 氧化亚铁硫杆菌, 驯化, 铀, 氟