Abstract：To increase the quality and yield of hyaluronan, a novel pretreatment process aiming at removing microorganism and protein in fermentation broth was developed. The optimal conditions for pretreatment process were obtained as follows: the concentration of trichloracetic acid was 25 g/L, the mixed diatomite loading (ratio of coarse to small diatomite 1.5:1) was 1.2% of fermentation broth, the filtration temperature was 60℃, and pH value was 7.0. After pretreatment, the hyaluronan solution was treated by postprocessing of precipitation with ethanol, air flotation and granulation by ethonal. The relative molecular weight of hyaluronan product was up to 1.9 million. 46.39% glucuronic acid and only 0.047% protein were found in the end product of hyaluronan. The yield of HA reached up to 91.3%.
邓禹;堵国成;李秀芬;刘登如;陈坚. 基于发酵液特性的透明质酸提取预处理工艺[J]. , 2007, 7(2): 380-384.
DENG Yu;DU Guo-cheng;LI Xiu-fen;LIU Deng-ru;CHEN Jian. A Pretreatment Process for Hyaluronan Extraction Based on Characteristics of Fermentation Broth. , 2007, 7(2): 380-384.
2007, Vol. 7 
