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›› 2007, Vol. 7 ›› Issue (2): 380-384.

• 生化工程专栏 • 上一篇    下一篇

基于发酵液特性的透明质酸提取预处理工艺

邓禹,堵国成,李秀芬,刘登如,陈坚   

  1. 江南大学 工业生物技术教育部重点实验室
  • 出版日期:2007-04-20 发布日期:2007-04-20

A Pretreatment Process for Hyaluronan Extraction Based on Characteristics of Fermentation Broth

DENG Yu,DU Guo-cheng,LI Xiu-fen,LIU Deng-ru,CHEN Jian   

  1. Key laboratory of industrial Biotechnology, Ministry of Education, Sourthern Yangtze university
  • Online:2007-04-20 Published:2007-04-20

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被引次数

50

摘要： 为进一步提高透明质酸成品质量和提取收率，针对透明质酸发酵液中菌体和蛋白质的物化特性，采用25 g/L三氯乙酸灭活菌体并使蛋白质变性沉淀，以发酵液质量1.2%的混合硅藻土(粗细硅藻土比为1.5:1)为过滤助剂，过滤温度60℃，pH 7.0的发酵液预处理工艺提取透明质酸成品. 再配以酒精沉淀、气浮、酒精造粒的后处理工艺，所得成品透明质酸中葡萄糖醛酸含量达46.39%，蛋白质含量仅为0.047%，分子量为190万，提取收率为91.3%.

关键词: 透明质酸提取, 预处理, 三氯乙酸, 硅藻土

Abstract: To increase the quality and yield of hyaluronan, a novel pretreatment process aiming at removing microorganism and protein in fermentation broth was developed. The optimal conditions for pretreatment process were obtained as follows: the concentration of trichloracetic acid was 25 g/L, the mixed diatomite loading (ratio of coarse to small diatomite 1.5:1) was 1.2% of fermentation broth, the filtration temperature was 60℃, and pH value was 7.0. After pretreatment, the hyaluronan solution was treated by postprocessing of precipitation with ethanol, air flotation and granulation by ethonal. The relative molecular weight of hyaluronan product was up to 1.9 million. 46.39% glucuronic acid and only 0.047% protein were found in the end product of hyaluronan. The yield of HA reached up to 91.3%.

Key words: hyaluronan extraction, pretreatment process, trichloracetic acid, diatomite