Preparation of Large Size Agarose Beads by Microporous Membrane Emulsification Technique
LIN Nan WU Jie ZHENG Guo-jun MA Guang-hui SU Zhi-guo
College of Life Science and Technology, Beijing University of Chemical Technology National Engineering Research Center for Biotechnology College of Life Science and Technology, Beijing University of Chemical Technology State Key Lab. Biochem. Eng., Inst. Process Eng., Chinese Academy of Sciences State Key Lab. Biochem. Eng., Inst. Process Eng., Chinese Academy of Sciences
Abstract：With 6% agarose solution as water phase, composed by liquid paraffin (LP) and petroleum ether (PE) with different volumetric ratios as oil phase, and PO-5S as emulsifier, agarose beads with average size of 90 mm were prepared by membrane emulsification, and the effects of pore size of the shirasu porous glass (SPG) membranes, oil phase composition, temperature and pressure on the size and size distribution of prepared agarose beads were investigated. The optimal preparation conditions were: the pore size of used SPG membrane was 25.9 mm, oil phase composed by volumetric ratio of LP and PE 11:1 and the preparation temperature 65℃. The prepared agarose beads with good reproducibility had an average diameter of 93.3 mm, size distribution index, Rspan, 1.25 and relative standard deviation 1.34%.
林楠 吴颉 郑国钧 马光辉 苏志国. 微孔膜乳化法制备大粒径琼脂糖微球[J]. , 2009, 9(5): 953-961.
LIN Nan WU Jie ZHENG Guo-jun MA Guang-hui SU Zhi-guo. Preparation of Large Size Agarose Beads by Microporous Membrane Emulsification Technique. , 2009, 9(5): 953-961.
2009, Vol. 9 
