微孔膜乳化法制备大粒径琼脂糖微球

›› 2009, Vol. 9 ›› Issue (5): 953-961.

微孔膜乳化法制备大粒径琼脂糖微球

林楠吴颉郑国钧马光辉苏志国

北京化工大学生命科学与技术学院国家生化工程技术研究中心北京化工大学生命科学与技术学院中国科学院过程工程研究所生化工程国家重点实验室中国科学院过程工程研究所生化工程国家重点实验室

收稿日期:2009-04-28 修回日期:2009-05-20 出版日期:2009-10-20 发布日期:2009-10-20
通讯作者: 林楠

Preparation of Large Size Agarose Beads by Microporous Membrane Emulsification Technique

LIN Nan WU Jie ZHENG Guo-jun MA Guang-hui SU Zhi-guo

College of Life Science and Technology, Beijing University of Chemical Technology National Engineering Research Center for Biotechnology College of Life Science and Technology, Beijing University of Chemical Technology State Key Lab. Biochem. Eng., Inst. Process Eng., Chinese Academy of Sciences State Key Lab. Biochem. Eng., Inst. Process Eng., Chinese Academy of Sciences

Received:2009-04-28 Revised:2009-05-20 Online:2009-10-20 Published:2009-10-20
Contact: LIN Nan

摘要/Abstract

摘要： 以6%的琼脂糖溶液为水相，不同体积配比的液体石蜡(LP)和石油醚(PE)的混合溶液为油相，PO-5S为乳化剂，采用微孔膜乳化法制备了平均粒径为90 mm的琼脂糖微球. 考察了SPG膜孔大小、油相组成、反应温度、压力等因素对成球粒径及其分布的影响. 结果表明，在使用膜孔为25.9 mm的微孔膜、LP/PE体积比为11:1及65℃的条件下可制得均一的大粒径琼脂糖微球，微球平均粒径为93.3 mm，粒度分布系数为1.25，且各批产品的相对标准偏差仅为1.34%，产品重复性良好.

关键词: 膜乳化, 琼脂糖微球, 大粒径, 粒径分布

Abstract: With 6% agarose solution as water phase, composed by liquid paraffin (LP) and petroleum ether (PE) with different volumetric ratios as oil phase, and PO-5S as emulsifier, agarose beads with average size of 90 mm were prepared by membrane emulsification, and the effects of pore size of the shirasu porous glass (SPG) membranes, oil phase composition, temperature and pressure on the size and size distribution of prepared agarose beads were investigated. The optimal preparation conditions were: the pore size of used SPG membrane was 25.9 mm, oil phase composed by volumetric ratio of LP and PE 11:1 and the preparation temperature 65℃. The prepared agarose beads with good reproducibility had an average diameter of 93.3 mm, size distribution index, Rspan, 1.25 and relative standard deviation 1.34%.

Key words: microporous membrane emulsification, agarose beads, large size, size distribution

中图分类号:

TB383

林楠吴颉郑国钧马光辉苏志国. 微孔膜乳化法制备大粒径琼脂糖微球[J]. , 2009, 9(5): 953-961.

LIN Nan WU Jie ZHENG Guo-jun MA Guang-hui SU Zhi-guo. Preparation of Large Size Agarose Beads by Microporous Membrane Emulsification Technique[J]. , 2009, 9(5): 953-961.

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