关键词: 交联琼脂糖凝胶, 表面修饰, 活化, N-羟基丁二酰亚胺, 烯丙基缩水甘油醚

Abstract: The homemade crosslinked agrose gel was first activated by allyl glycidyl ether, then reacted with mercaptoacetic acid and coupled with N-hydroxysuccinimide (NHS). Thus a kind of activated intermediate agarose gel with a 10-atoms-long arm and high specificity to amino-group was obtained. The modification density of NHS can be situated in a wide range of 20~150 mmol/mL by controlling the olefinic bond on the gel. This can reduce miscellaneous group coupling to the gel, and benefit for the ligand density design. The activated gel does not show any difference compared with original gel in physicochemical performance. The activated gel is easy to couple with L-phenylalanine in 30 min, and the coupling efficiency is greater than 90%. Moreover, the coupled ligands have been stable and without leaking during storage for 30 d.

Key words: crosslinked agarose gel, surface modification, activation, N-hydroxysuccinimide, allyl glycidyl ether