Interaction between Complexes of Chitosan with La3+ and Dy3+ and Bovine Serum Albumin
WU Jin-xiu LIU Zhao-gang HU Yan-hong WANG Mi-tang
College of Rare earth, Inner Mongolia University of Science and Technology College of Rare earth, Inner Mongolia University of Science and Technology Inner Mongolia Science and Technology University Material institute Inner Mongolia Science and Technology University Material institute Inner Mongolia Science and Technology University Material institute
Abstract：The interaction between chitosan (CS), its complexes CS-La and CS-Dy, and bovine serum albumin (BSA) in vitro under simulative physiological conditions was studied by fluorescence, ultraviolet-visible absorption and synchronous fluorescence spectrometry. The results showed that CS, CS-La, CS-Dy had strong effects on quenching of the fluorescence launching and enhanced the UV absorption spectra of BSA. After the fluorescence quenching, the obtained data were analyzed by Stern-Volmer equation, the results indicated that the reactions between BSA and CS, CS-La and CS-Dy generated new complexes. The quenching belonged to static fluorescence quenching, with non-radiation energy transfer happening within single molecule, but the static and dynamic quenching coexisted in the reactions between CS and CS-Dy and BSA. The binding constant KA (291 K, CS-BSA: 1.807′104 [L/(g·s)], CS-La-BSA: 3.065′104 [L/(g·s)], CS-Dy-BSA: 2.193′104 [L/(g·s)]; 310 K, CS-BSA: 2.665′104 [L/(g·s)], CS-La-BSA: 2.022′104 [L/(g·s)], CS-Dy-BSA: 7.246′104 [L/(g·s)] and thermodynamics parameters (DH, DS, DG) were calculated, respectively according to the equation of fluorescence spectrometry at different temperatures. Based on thermodynamic data, the main reactions between CS and CS-Dy and BSA were electrostatic and hydrophobic forces, but those between CS-La and BSA van der Waals and hydrogen bond forces. The effects of CS, CS-La and CS-Dy on the conformation of BSA shown by synchronous fluorescence spectrometry indicated that they changed the micro-environment and conformation of BSA molecules.
吴锦绣 李梅 柳召刚 胡艳宏 王觅堂. La3+和Dy3+壳聚糖配合物与牛血清白蛋白相互作用[J]. 过程工程学报, 2016, 16(2): 279-285.
WU Jin-xiu LIU Zhao-gang HU Yan-hong WANG Mi-tang. Interaction between Complexes of Chitosan with La3+ and Dy3+ and Bovine Serum Albumin. Chin. J. Process Eng., 2016, 16(2): 279-285.
2016, Vol. 16 
