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›› 2009, Vol. 9 ›› Issue (1): 113-117.

• 生化工程专栏 • 上一篇    下一篇

甲烷氧化混合菌群MY9的生长特性

罗明芳 吴昊 王磊 邢新会 邢新会   

  1. 中国科学院研究生院化学与化学工程学院 清华大学化工系生物化工研究所 大连理工大学先进控制技术研究所 清华大学化工系生物化工研究所
  • 收稿日期:2008-07-21 修回日期:2008-09-27 出版日期:2009-02-20 发布日期:2009-02-20
  • 通讯作者: 罗明芳

Growth Characteristics of a Methane-utilizing Mixed Consortia MY9

LUO Ming-fang WU Hao WANG Lei YANG Cheng XING Xin-hui   

  1. Institute of Biochemical Engineering, Department of Chemical Engineering, Tsinghua University Institute of Advanced Control Technology, Dalian University of Technology Department of Chemical Engineering, Tsinghua University
  • Received:2008-07-21 Revised:2008-09-27 Online:2009-02-20 Published:2009-02-20
  • Contact: LUO Ming-fang

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被引次数

12

摘要： 考察了铜离子浓度对甲烷氧化混合菌群MY9(CGMCC No.1893)生长和甲烷单加氧酶活性的影响，并对混合菌群中的非甲烷氧化菌进行了分离鉴定和碳源特异性研究. 结果表明，甲烷氧化混合菌群MY9能利用甲醇、乙醇、丁醇、异丙醇、甘油等多种非甲烷化合物作为生长碳源，且具有耐高Cu2+浓度的生长特性. 从混合菌群中分离的非甲烷氧化菌Acinetobacter junii No.8能分别以4%甲醇、1%乙醇或0.6%二氯甲烷作为唯一生长碳源，适用于高浓度甲醇废水、乙醇废水或含二氯甲烷废水的生物处理.

关键词: 甲醇, 甲烷氧化菌, 甲烷氧化混合菌群, Methylosinus trichosporium

Abstract: The growth characteristics of a methane-utilizing mixed consortia MY9 (CGMCC No.1893) were examined with different copper ion concentrations and carbon sources. Some heterotrophs of the mixed consortia were isolated and characterized. The results showed that the mixed consortia could utilize many organic chemicals such as methanol, ethanol, butanol, isopropanol and glycerol as the carbon source as well as methane. It could also grow in the condition of high concentration of copper. One of the heterotrophs isolated from the mixed consortia, identified as Acinetobacter junii No.8, could utilize 4% methanol or 1% ethanol or 0.6%(j) dichloromethane as a sole carbon source. These results suggest that the methane-utilizing mixed consortia MY9 or A. junii No.8 can be potentially used in the biocatalytical treatment of wastewater containing high concentrations of methanol wastewater, ethanol or dichloromethane.

Key words: methanol, methanotrophs, methane-utilizing mixed culture, Methylosinus trichosporium

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