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›› 2008, Vol. 8 ›› Issue (3): 583-588.

• 材料工程专栏 • 上一篇    下一篇

磁性SiO2纳米粒子的制备及其用于漆酶固定化

刘宇 王峰 郭晨 刘春朝 刘会洲   

  1. 中国科学院过程工程研究所绿色过程与工程重点实验室 中国科学院过程工程研究所生化工程国家重点实验室 中科院过程工程研究所分离科学与工程青年实验室、生化工程国家重点实验室、中科院研究生院 中国科学院过程工程研究所生化工程国家重点实验室 中国科学院过程工程研究所分离工程与工程青年实验室
  • 收稿日期:2008-04-11 修回日期:2008-04-25 出版日期:2008-06-20 发布日期:2008-06-20
  • 通讯作者: 刘宇

Preparation of Magnetic Silica Nanoparticles and Their Application in Laccase Immobilization

LIU Yu GUO Chen WANG Feng LIU Chun-zhao LIU Hui-zhou   

  1. Key Laboratory of Green Process and Engineering, Institute of Process Engineering, Chinese Academy of Sciences State Key Laboratory of Biochemical Engineering, Institute of Process Engineering , Chinese Academy of Sciences Young Scientist Laboratory of Separation Science and Engineering, State Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences State Key Laboratory of Biochemical Engineering, Institute of Process Engineering Institute of Process Engineering, Chinese Academy of Sciences
  • Received:2008-04-11 Revised:2008-04-25 Online:2008-06-20 Published:2008-06-20
  • Contact: LIU Yu

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摘要： 以氨基修饰的磁性SiO2纳米粒子为载体，通过交联剂戊二醛固定漆酶，对固定化条件进行了优化，比较了固定化酶与游离酶的酶学性质. 结果表明，漆酶固定化的最佳条件为戊二醛浓度8%(w)，固定化时间6 h，缓冲液pH值7.0，初始酶液浓度0.15 g/L. 固定化的漆酶的最适pH为4.0，最适温度为20℃. 在60℃条件下保温4 h，固定化漆酶仍能保持酶活力60.9%，在连续10次操作后，酶活力仍能保持55%以上，其热稳定性和操作稳定性均比游离酶高.

关键词: 磁性SiO2, 纳米粒子, 漆酶, 固定化

Abstract: Laccase was immobilized on the silanized magnetic silica nanoparticles by using glutaradehyde as the cross-linker. Immobilization of laccase was investigated and its immobilizing conditions were optimized. The results were achieved with the concentration of 8% glutaradehyde, immobilizing time of 6 h, the buffer of pH 7.0 and initial laccase concentration of 0.15 g/L. The optimum pH and temperature of immobilized laccase were 4.0 and 20℃, respectively. After the immobilized enzyme was kept at 60℃ for 4 h, it remained 60.9% of its initial activity. The immobilized laccase could retain above 55% of activity after 10 consecutive operations. The experimental results showed that the thermal stability and operational stability of immobilized laccase were improved in comparison with free laccase.

Key words: magnetic silica, nanoparticle, laccase, immobilization

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