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过程工程学报 ›› 2016, Vol. 16 ›› Issue (3): 500-504.DOI: 10.12034/j.issn.1009-606X.215298

• 生化工程专栏 • 上一篇    下一篇

无溶剂体系中共吸附固定化脂肪酶催化合成植物甾醇酯

周海燕 蒋振华 任立伟 于敏 周华 韦萍   

  1. 南京工业大学生物与制药工程学院 南京工业大学生物与制药工程学院 南京工业大学生物与制药工程学院 南京工业大学生物与制药工程学院 南京工业大学药学院 南京工业大学制药与生命科学学院
  • 收稿日期:2015-08-19 修回日期:2016-02-01 出版日期:2016-06-20 发布日期:2017-04-28
  • 通讯作者: 周华

Synthesis of Phytosterol Esters Catalyzed by Immobilized Lipase in Solvent-free System

ZHOU Hai-yan JIANG Zhen-hua REN Li-wei YU Min ZHOU Hua WEI Ping   

  1. College of Pharmacy and Life Science，Nanjing University of Technology College of Pharmacy and Life Science，Nanjing University of Technology College of Pharmacy and Life Science，Nanjing University of Technology College of Pharmacy and Life Science，Nanjing University of Technology College of Pharmacy and Life Science，Nanjing University of Technology College of Life Science and Pharmacy, Nanjing University of Technolog
  • Received:2015-08-19 Revised:2016-02-01 Online:2016-06-20 Published:2017-04-28
  • Contact: ZHOU Hua

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摘要： 以大孔树脂NKA吸附固定化脂肪酶为催化剂，以月桂酸和植物甾醇为反应物，在无溶剂体系中合成植物甾醇酯；以NKA为载体共吸附固定葡聚糖与酶，考察了反应温度、底物摩尔比、糖种类及葡聚糖添加量和反应时间对酶催化性能的影响. 结果表明，在酸/醇摩尔比6:1、葡聚糖与酶共同吸附固定时分子量为10 kDa的葡聚糖添加量为酶量7.5%的条件下，60℃下反应12 h，酯化率高达96.2%，酯化反应时间比简单吸附固定化酶缩短4 h.

关键词: 脂肪酶, 无溶剂体系, 固定化, 植物甾醇酯

Abstract: The aim of this work is to synthesize lauric acid phytosterol esters by lipase in solvent-free system. The lipase was immobilized on macroporous resin NKA, with addition of dextran. The reaction conditions were optimized. The results showed that under the optimized conditions of a molar ratio of lauric acid to phytosterol of 6:1, 7.5% dextra of molecular weight 10 kDa and the reaction temperature of 60℃ in solvent-free system, the esterification rate reached 96.2% after 12 h. The reaction time of the synthess of phytosterol esters decrease 4 h comparing with the immobilized lipase on a macroporous resin.

Key words: lipase, solvent-free system, immobilization, phytosterol ester

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