关键词: 固定化, 东北红豆杉细胞, 细胞生长, 细胞代谢

Abstract: Alginate-chitosan beads with liquid cores as a novel carrier was introduced to immobilize Taxus cuspidata cells in this work. Physiology of Taxus cuspidata cells enclosed in alginate-chitosan liquid or solid core beads were investigated. It was found that the cell viability was greater in liquid core beads than in solid ones. Liquid core beads enhance the absorption of sucrose and cell metabolism. Intracellular starch granules became gradually less according to the order of solid beads, liquid core beads and suspension. Both alginate-chitosan liquid core and solid core beads improved cell permeability, especially liquid core beads. This benefited the release of secondary metabolite and other intracellular substances into the culture medium. The synthesis and secretion of phenolics increased. Overally, the growth and metabolism of Taxus cuspidata cells encapsulated in alginate-chitosan liquid core beads were improved than in solid beads. The results show that liquid core beads is a better carrier for plant cell immobilization.

Key words: immobilization, Taxus cuspidata cell, cell growth, cell metabolism