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›› 2006, Vol. 6 ›› Issue (4): 623-626.

• 生化工程专栏 • 上一篇    下一篇

氨基酸促进甘油合成的动力学分析

谢涛,方慧英,诸葛健   

  1. 北京化工大学环境工程系
  • 出版日期:2006-08-20 发布日期:2006-08-20

Kinetic Analysis of Stimulatory Effects of Amino Acids on Glycerol Formation

XIE Tao,FANG Hui-ying,ZHUGE Jian   

  1. Department of Environmental Engineering Beijing University of Chemical Engineering
  • Online:2006-08-20 Published:2006-08-20

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被引次数

1

摘要： 在产甘油假丝酵母分批发酵生产甘油的过程中，考察了添加甘氨酸、谷氨酸、天冬酰胺、丙酮酸和a-酮戊二酸等物质对菌体生长、维持代谢、甘油生成及副产物形成的影响. 结果表明，在发酵全过程中，它们对菌体的生长基本没有影响，但能加快葡萄糖的消耗速率、缩短发酵周期、提高甘油得率，从而使甘油的生产强度分别达到1.66, 1.59, 1.61, 1.54和1.68 g/(L×h)，比对照分别提高了25.18%, 19.21%, 20.77%, 15.92%和26.32 %；副产物的形成主要发生在快速生长阶段，而此阶段用于菌体生长、维持代谢和甘油合成所消耗的葡萄糖基本没有变化；在稳定生长期，它们可以减少用于菌体生长、维持代谢和形成副产物的葡萄糖消耗量，导致流向甘油合成途径的葡萄糖代谢流增加.

关键词: 氨基酸, 中间代谢物, 产甘油假丝酵母, 甘油合成, 动力学分析

Abstract: The effects of glycin, glutamic acid, asparagin, pyruvic acid and a-oxoglutarate as additives on cell growth, maintenance, byproducts formation and glycerol production by C. glycerinogenes were investigated. The results showed that the addition of the above substances had little influence on cell growth during the whole fermentation process. However, it could hasten the glucose consumption rate, shorten the fermentation time, and enhance the glycerol yield on glucose. The glycerol productivities reached 1.66, 1.59, 1.61, 1.54 and 1.68 g/(L×h), respectively. As compared with the control, the glycerol productivities increased 25.18%, 19.21%, 20.77%, 15.92% and 26.32%, respectively. Further analysis indicated that the byproduct was mainly produced during the rapid growth phase, where the glucose consumption in cell growth, maintenance and glycerol production had little change. During the steady growth phase, the glucose consumption for cell growth, maintenance and byproducts formation decreased, thus resulting in the incremental carbon metabolic flux toward glycerol biosynthesis pathway.

Key words: amino acid, intermediate metabolites, Candida glycerinogene, glycerol formation, kinetic analysis