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›› 2008, Vol. 8 ›› Issue (4): 779-783.

• 生化工程专栏 • 上一篇    下一篇

2,3-丁二醇发酵液的絮凝除菌与絮凝细胞的循环利用

张江红 孙丽慧 修志龙   

  1. 大连普兰店市经济开发区渤海路8号奇运生制药有限公司 大连理工大学环境与生命学院生物科学与工程系 大连理工大学环境与生命学院生物科学与工程系
  • 收稿日期:2008-03-18 修回日期:2008-04-28 出版日期:2008-08-20 发布日期:2008-08-20
  • 通讯作者: 修志龙

Removal of Klebsiella pneumoniae Cells from 2, 3-Butanediol Fermentation Broth by Flocculation and Reuse of Cells in Flocs

ZHANG Jiang-hong SUN Li-hui XIU Zhi-long   

  1. Department of Bioscience and Biotechnology, School of Environmental and Biological Science and Technology, Dalian University of Technology Department of Bioscience and Biotechnology, School of Environmental and Biological Science and Technology, Dalian University of Technology Department of Bioscience and Biotechnology, School of Environmental and Biological Science and Technology, Dalian University of Technology
  • Received:2008-03-18 Revised:2008-04-28 Online:2008-08-20 Published:2008-08-20
  • Contact: XIU Zhi-long

摘要: 研究了用壳聚糖/海藻酸钠复合絮凝剂处理2,3-丁二醇发酵液的工艺条件,以絮凝率为指标,考察了壳聚糖分子量、壳聚糖用量、海藻酸钠助凝剂用量、发酵液pH值、搅拌时间等因素对处理效果的影响,确定了适于2,3-丁二醇发酵液体系的絮凝工艺. 结果表明,最佳操作条件为壳聚糖分子量40 kDa,壳聚糖用量0.375 g/L,海藻酸钠助凝剂用量0.250 g/L,发酵液pH 5.0,搅拌时间30 min,静置1 h. 该条件下,絮凝率可达98%以上,2,3-丁二醇保留率约为99%,且絮凝后上清液清澈、透明. 絮凝后的菌体可再次利用,发酵过程中菌体最高浓度(OD值)可达13.5,其转化能力与絮凝前相当.

关键词: 3-丁二醇, 絮凝, 壳聚糖, 发酵

Abstract: The pretreatment of 2,3-butanediol fermentation broth by chitosan flocculation was investigated. Using sodium alginate as coagulant, the effects of chitosan molecular weight, chitosan dosage, coagulant dosage, pH and turbidity time on the flocculation of fermentation broth were investigated, respectively. The performance was evaluated by flocculation ratio. According to the results, the optimum flocculation conditions for Klebsiella pneumoniae cells were as follows: 0.375 g/L of chitoson with 40 kDa molecular weight, 0.250 g/L of sodium alginate, pH 5.0, 30 min of stirring time and 1 h of settlement time. The flocculation rate reached 98%, the retained ratio of 2,3-butanediol reached 99%, and the supernatant in the broth was clear. After flocculation the cells of Klebsiella pneumoniae in the flocs grew well (OD 13.5) and were used repeatedly.

Key words: 3-butanediol, flocculation, chitosan, fermentatio

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