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›› 2013, Vol. 13 ›› Issue (5): 870-876.

• 生化工程专栏 • 上一篇    下一篇

mPEG-PLGA多孔微球的制备及其对降钙素的吸附行为

张慧霞 王玉霞 韦祎 阮燕晔 马光辉   

  1. 沈阳农业大学 中国科学院过程工程研究所生化工程国家重点实验室 中国科学院过程工程研究所生化工程国家重点实验室 Shenyang Agriculture University 中国科学院过程工程研究所生化工程国家重点实验室
  • 收稿日期:2013-05-22 修回日期:2013-06-28 出版日期:2013-10-20 发布日期:2013-10-20
  • 通讯作者: 张慧霞

Preparation of mPEG-PLGA Porous Microspheres and Their Adsorption to Salcatonin

ZHANG Hui-xia WANG Yu-xia WEI Yi RUAN Yan-ye MA Guang-hui   

  1. Shenyang Agriculture University State Key Lab of Biochemical Engineering, Institute of Process Engineering, CAS State Key Lab of Biochemical Engineering, Institute of Process Engineering, CAS 沈阳农业大学 State Key Lab. Biochem. Eng., Inst. Process Eng., Chinese Academy of Sciences
  • Received:2013-05-22 Revised:2013-06-28 Online:2013-10-20 Published:2013-10-20
  • Contact: ZHANG Hui-xia

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被引次数

1

摘要： 以聚乙二醇-聚(乳酸-乙醇酸)共聚物(mPEG-PLGA)为材料，采用溶剂挥发法与快速膜乳化法制备了尺寸均一的mPEG-PLGA多孔微球，用其吸附降钙素，考察了降钙素浓度、吸附时间、离子浓度、温度及pH值及微球性质对吸附的影响. 结果表明，优化的吸附条件为：降钙素浓度1.0 mg/mL, pH 7.4, NaCl浓度0.2 mol/L, 14℃下吸附8 h，该条件下，微球吸附量为48.9 mg/g；吸附量与微球比表面积正相关.

关键词: mPEG-PLGA, 多孔微球, 降钙素, 吸附, 比表面积

Abstract: Relatively uniform size porous methoxy poly(ethylene glycol-L-lactic-co-glycolic acid) (mPEG-PLGA) microspheres were successfully prepared by premix membrane emulsification with solvent evaporate method. The effects of salcatonin concentration, salt concentration, pH value, adsorption temperature and time, and properties of microspheres on the adsorption capacity of salcatonin were investigated. The results showed that the adsorption capacity of porous microspheres reached 48.9 mg/g under the conditions of 1.0 mg/mL salcatonin concentration, pH value 7.4 of phosphate buffer, 0.2 mol/L NaCl concentration, 14℃ adsorption temperature and 8 h adsorption time. In addition, the adsorption capacity increased with the increase of specific surface area.

Key words: methoxy poly(ethylene glycol-L-lactic-co-glycolic acid), porous microspheres, salcatonin, adsorption, specific surface are

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