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›› 2005, Vol. 5 ›› Issue (3): 255-259.

• 反应与分离 • 上一篇    下一篇

CTAB混合反胶团萃取工业脂肪酶

沈睿,刘俊果,邢建民,刘会洲   

  1. 中国科学院过程工程研究所分离科学与工程实验室
  • 出版日期:2005-06-20 发布日期:2005-06-20

Extraction of Industrial Lipase with CTAB-based Mixed Reverse Micelles

SHEN Rui,LIU Jun-guo,XING Jian-min,LIU Hui-zhou   

  1. Lab. Sep. Sci. & Eng., Institute of Process Engineering, Chinese Academy of Sciences
  • Online:2005-06-20 Published:2005-06-20

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摘要： 研究了CTAB与Tween、含氧有机物形成的混合反胶团对工业脂肪酶进行萃取分离的效果. 实验表明，CTAB-Tween85和CTAB-含氧有机物混合反胶团的萃取率高于单一CTAB反胶团；反萃时CTAB-含氧有机物混合反胶团的反萃率与单一CTAB反胶团的反萃率相似，CTAB-Tween60和Tween40混合反胶团的反萃效果优于单一CTAB反胶团. 通过测定反萃水相的酶活，发现CTAB-TRPO混合反胶团的效果最好，酶活回收率最高，可以达到70%.

关键词: CTAB, 脂肪酶, Tween, 混合反胶团, TRPO, TBP, 乙酸丁酯

Abstract: The extraction of industrial lipase with mixed reverse micelles formed by CTAB with Tween, TRPO, TBP and butyl acetate was investigated. The transfer of industrial lipase to the mixed reverse micelles formed from CTAB with Tween85, TRPO, TBP, butyl acetate was higher than that of the reverse micelles formed with CTAB reverse micelles. The addition of TRPO, TBP and butyl acetate to the CTAB reverse micelles had no effect on the backward transfer of industrial lipase. The mixed reverse micelles formed from CTAB with Tween60, Tween40 could obviously enhance the industrial lipase backward transfer. The activity recovery of industrial lipase with the mixed reverse micelles formed from CTAB with TRPO was about 70%, which was the best in these mixed reverse micelles tested.

Key words: CTAB, lipase, Tween, mixed reverse micelles, TRPO, TBP, butyl acetate