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›› 2002, Vol. 2 ›› Issue (4): 0-0.

• 4 •    

用化学交联法制备聚(4-乙烯基吡啶)纳米凝胶微球

马光辉   

  1. 中国科学院过程工程研究所生化工程国家重点实验室,北京 100080
  • 出版日期:2002-08-20 发布日期:2002-08-20

Preparation of Microgel by Crosslinking Partially Quaternized Poly(4-Vinyl Pyridine)

MA Guang-hui   

  1. State Key Lab. Bioreactor Eng., East China Univ. Sci. & Technol., Shanghai 200237, China
  • Online:2002-08-20 Published:2002-08-20

摘要: 在1,1,2-三氯乙烷/甲醇混合溶剂中用碘代甲烷使聚(4-乙烯基吡啶)(P4VP)线性高分子部分季铵化后,在水中制成微乳液,并用1,4-二碘丁烷交联剂对P4VP微乳液内部进行化学交联,得到固化凝胶微球. 考察了部分季铵化比率、交联温度以及聚合物浓度对微球直径和直径分布的影响,发现微球直径随季铵化比率的增加、交联温度的增加及聚合物浓度的减少而减小. 当季铵化比率为75%(摩尔比)、交联温度为60℃、聚合物浓度为1.0%(w)时,得到了直径为20 nm、粒径分布为±15%的凝胶微球. 本研究的重要特征是不加入任何乳化剂也能得到很小的纳米凝胶微球.

关键词: 聚(4-乙烯基吡啶), 纳米凝胶微球, 部分季铵化, 微乳液, 交联反应

Abstract: The kinetic model of glucose metabolism was established and successfully applied to batch cultures of rCHO and rBHK cells. It was found that a large amount of glucose was utilized for cell maintenance, and the overwhelming majority of maintenance energy from glucose was by its anaerobic metabolism in both rBHK and rCHO cell cultures. The overall maintenance coefficients from aerobic metabolism were 1.9′10-13 mmol/(cell·h) for rCHO cells and 7′10-13 mmol/(cell·h) for rBHK cells. In addition, all GO/T and EO/T gradually increased with the same trend as the cell growth in the culture of both rCHO and rBHK cells. The overall molecule yield coefficients of lactate to glucose were 1.61 for rCHO cells and 1.38 for rBHK cells. The yield coefficients of cell to glucose were 4.5′108 cells/mmol for rCHO cells and 1.9 ′108 cells/mmol for rBHK cells, respectively.

Key words: poly(4-vinyl pyridine), nano-gel, partially-quaternized, micro-emulsion, crosslinking

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