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过程工程学报 ›› 2016, Vol. 16 ›› Issue (4): 601-606.DOI: 10.12034/j.issn.1009-606X.215434

• 反应与分离 • 上一篇    下一篇

Amberlite IRC-50树脂吸附与解吸-聚赖氨酸的过程优化

高阳1,何洪刚1,李芳良1,袁光杰1,陈旭升1,毛忠贵2   

  1. 1. 江南大学生物工程学院工业生物技术教育部重点实验室
    2. 江南大学生物工程学院
  • 收稿日期:2015-12-30 修回日期:2016-02-19 出版日期:2016-08-20 发布日期:2016-10-10
  • 通讯作者: 毛忠贵

Optimization and Evaluation on Adsorption and Desorption of ?-Poly-L-lysine on Amberlite IRC-50 Resins

GAO Yang 1,HE Hong-gang 1,LI Fang-liang 1,YUAN Guang-jie 1,CHEN Xu-sheng 1,MAO Zhong-xian 2   

  1. 1. School of Biotechnology, Jiangnan University
    2. Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University
  • Received:2015-12-30 Revised:2016-02-19 Online:2016-08-20 Published:2016-10-10
  • Contact: MAO Zhong-xian

摘要: 实验考察了氢型、钠型、氨型大孔弱酸性树脂Amberlite IRC-50吸附与解吸?-聚赖氨酸(?-PL)的条件,并研究了3种树脂的动态吸附与洗脱过程. 静态实验结果表明,3种树脂吸附?-PL的最适pH值均为8.0,吸附量分别为238.7, 293.3和298.1 mg/g;3种树脂的最佳洗脱剂浓度均为0.1 mol/L,洗脱率均达到96%以上;钠型和氨型树脂吸附所得?-PL纯度比氢型树脂提高12%,吸附速率常数是氢型树脂的近2倍,更适用于?-PL的分离与提取.

关键词: ?-聚赖氨酸, 大孔弱酸树脂, 吸附, 动力学

Abstract: The adsorption capacity and elution rate of H+-form and Na+-form as well as NH4+-form Amberlite IRC-50 (IRC-50) resins for ?-poly-L-lysine (?-PL) at different pH values and eluant concentrations were evaluated by static adsorption experiment. Meanwhile, the dynamic adsorption and elution processes of three forms of IRC-50 resins were also studied. The results revealed that the optimum pH value of three forms of IRC-50 resins for ?-PL adsorption was 8.0, the saturated adsorption capacity were 238.7, 293.3 and 298.1 mg/g, respectively. In addition, the optimum concentration of the eluent for three forms of IRC-50 resin was 0.1 mol/L, and the elution rate reached more than 96%. The results of dynamic adsorption and elution experiments indicated that the purity of ε-PL obtained by Na+-form and NH4+-form resins were 12% higher than that of H+-form resin. Moreover, the kinetic adsorption rate constants for Na+-form and NH4+-form resins are nearly 2 times more than that of H+-form resin. Therefore, Na+-form and NH4+-form resins are more suitable for the separation and extraction of ?-PL than H+-form resin.

Key words: ?-poly-L-lysine, macroporous weak acid resin, adsorption, kinetics

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