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过程工程学报 ›› 2017, Vol. 17 ›› Issue (2): 389-394.DOI: 10.12034/j.issn.1009-606X.216334

• 材料工程 • 上一篇    下一篇

牛煅烧骨/I型胶原复合骨修复材料制备及评价

  雷雄心1, 李赛娜2, 康跻耀3, 彭公泽4,  张贵锋3*   

  1. 1. 北京中医药大学中药学院,北京 100010
    2. 山东农业大学食品学院,山东泰安 271018
    3. 中国科学院过程工程研究所,北京 100190
    4. 南方医科大学珠江医院,广东 广州 510280
  • 收稿日期:2016-10-26 修回日期:2016-12-08 出版日期:2017-04-20 发布日期:2017-04-19
  • 通讯作者: 张贵锋
  • 基金资助:
    国家高技术研究发展计划(863);广东省自然科学基金资助项目

Preparation and Evaluation of True Bone Ceramic/Type I Collagen Composite Bone Repair Materials

  LEI Xiong-xin1,  LI Sai-na2,  KANG Ji-yao3,  PENG Gong-ze4,  ZHANG Gui-feng3*   

  1. 1. College of Traditional Chinese Medicine, Beijing University of Chinese Medicine, Beijing 100102, China;
    2. Institute of Food, Shandong Agricultural University, Taian, Shandong 271018, China; 
    3. Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, China;
    4. Zhujiang Hospital, Southern Medical University, Guangzhou, Guangdong 510280, China
  • Received:2016-10-26 Revised:2016-12-08 Online:2017-04-20 Published:2017-04-19
  • Contact: ZHANG Gui-feng

摘要: 研究了基于牛煅烧骨/Ⅰ型胶原(TBC/CoL)复合材料的骨组织工程支架制备方法,并对其理化性能和细胞相容性进行评价。将TBC/CoL按照不同比例混合,经戊二醛交联,冷冻干燥等处理后制得复合支架,研究不同材料比例及胶原交联对材料性能的影响;采用扫描电镜(SEM)、X射线衍射(XRD)、傅里叶红外光谱(FIRT)和差示扫描量热(DSC-TGA)等对支架材料的理化性能进行分析;通过CCK-8法评价细胞在支架上增殖情况。SEM结果表明制备的TBC/CoL支架具有多孔结构,平均孔隙率93.5%,杨氏模量为4.36±0.21 MPa;XRD和FTIR谱图结果表明复合支架中的TBC在CoL上发生了化学吸附,CoL结构保持完整;MC3T3-E1细胞在支架上培养24h后,SEM可见细胞在支架表面生长良好。CCK-8法检测结果表明细胞在支架上增殖情况与对照组无明显差别(P>0.05)。表明将牛煅烧骨和Ⅰ型胶原复合制备的支架具有良好的孔隙率,较好的机械强度,良好的细胞相容性和细胞增殖特性。

关键词: 牛煅烧骨, I型胶原, 骨组织工程, 支架材料

Abstract: True Bone Ceramic/ type I collagen (TBC/CoL) composite scaffolds was prepared, and its physicochemical properties and cell compatibility were evaluated. The TBC and CoL was mixed with different ratio and collagen crosslinked by glutaraldehyde. The heterogeneous mixture was freeze-dried and the dehydrated state was obtained as the scaffold. The effects of different materials and cross-linking of collagen on the scaffold properties were investigated. The physical and chemical properties of the scaffolds were analyzed by scanning electron microscopy (SEM), X-ray diffraction (XRD), fourier transform infrared spectroscopy (FIRT) and differential scanning calorimetry (DSC-TGA). The proliferation of the cells on the scaffold was evaluated by the CCK-8 method. SEM results show that the prepared TBC/CoL scaffolds have a porous structure with an average porosity of 93.5% and a Young's modulus of 4.36 ± 0.21 MPa. XRD and FTIR spectra indicated that TBC in the composite scaffolds were chemically adsorbed on the CoL matrix and the CoL structure in the matrix remained intact. MC3T3-E1 cells were cultured on the scaffold. After 24 h of culture, the cells grew well on the scaffold. CCK-8 assay showed that the proliferation of cells on the scaffold was not significantly different from the control group (p>0.05). The results showed that the scaffold prepared by True Bone Ceramic and type I collagen had good porosity, good mechanical strength, good cell compatibility and cell proliferation.

Key words: True Bone Ceramic, Type I collagen, Bone tissue engineering, Scaffold