欢迎访问过程工程学报, 今天是

过程工程学报 ›› 2024, Vol. 24 ›› Issue (1): 79-86.DOI: 10.12034/j.issn.1009-606X.222462

• 研究论文 • 上一篇    下一篇

表面改性PET网状纤维支架材料制备及其生物安全性评价

李阳1,2,3， 高建萍1,2， 张扬1， 彭青3， 高毅3， 张贵锋1,2*   

  1. 1. 中国科学院过程工程研究所生化工程国家重点实验室，北京 100190 2. 中国科学院大学化学工程学院，北京 100049 3. 南方医科大学珠江医院再生医学研究所，广东 广州 510282
  • 收稿日期:2022-12-23 修回日期:2023-04-12 出版日期:2024-01-28 发布日期:2024-01-26
  • 通讯作者: 张贵锋 gfzhang@ipe.ac.cn
  • 基金资助:
    国家重点研发计划;国家重点研发计划;国家重点研发计划;国家自然科学基金

Preparation and biological safety evaluation of surface modified PET reticular fiber scaffold materials

Yang LI1,2,3,  Jianping GAO1,2,  Yang ZHANG1,  Qing PENG3,  Yi GAO3,  Guifeng ZHANG1,2*   

  1. 1. National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, China 2. School of Chemical and Engineering, University of Chinese Academy of Sciences, Beijing 100049, China 3. Institute of Regenerative Medicine, Zhujiang Hospital, Southern Medical University, Guangzhou, Guangdong 510282, China
  • Received:2022-12-23 Revised:2023-04-12 Online:2024-01-28 Published:2024-01-26
  • Contact: ZHANG Gui-feng gfzhang@ipe.ac.cn
  • Supported by:
    the National Key R&D Program of China;the National Key R&D Program of China;the National Key R&D Program of China;the National Natural Science Foundation of China

RichHTML

PDF

摘要： 以氨气作为等离子体气体，对合成高分子聚对苯二甲酸乙二醇酯(PET)支架材料表面进行低温等离子体处理，获得了一种亲水性和生物相容性得到改善的网状纤维支架材料PET-LTPT。通过XPS光电子能谱仪、水接触角、电镜、BET比表面积测定对材料进行物理化学表征，同时培养肝细胞测定其生物相容性，并对其开展体外细胞毒性、皮内反应试验以及皮肤致敏试验以评估其生物安全性。结果表明，低温等离子体处理后，PET材料引入了N元素，含量为2.38wt%，亲水性得到了显著改善(P<0.001)，比表面积为0.37 m2/g。PET-LTPT生物安全性良好，人肝癌细胞C3A细胞在处理后的PET-LTPT材料上实现黏附和增殖，证明了表面改性PET网状纤维支架材料用于生物人工肝用肝组织化三维培养具有可行性。

关键词: PET, 低温等离子体, 生物安全性评价, 人工肝, 三维培养支架材料

Abstract: Using ammonia as plasma gas, the surface of synthetic polymer polyethylene terephthalate (PET) scaffold material was treated with low-temperature plasma, and a kind of reticular fiber scaffold material with improved hydrophilicity and biocompatibility was obtained. The material was characterized physically and chemically by XPS photoelectron spectroscopy, water contact angle, electron microscopy, and BET-specific surface area measurement. At the same time, hepatocytes were cultured to measure their biocompatibility, and in vitro cytotoxicity, intradermal reaction, and skin sensitization tests were carried out to evaluate their biological safety. The results showed that after low-temperature plasma treatment, N element was introduced into PET material. Compared with that before treatment, the percentage of O/C elements decreased by 2.05 percentage points, and the percentage of N/C elements increased by 2.10 percentage points. The water contact angle decreased from 123.32° to 30.55°, and the hydrophilicity was significantly improved. The surface roughness of PET material was increased after plasma treatment, and the specific surface area of the material was 0.37 m2/g. Plasma treatment can improve the biocompatibility of PET materials and promote C3A cell adhesion and proliferation. Compared with the untreated group, the treated group showed better biological activity. At the same time, PET-LTPT had good biological safety. MTT cytotoxicity assay in vitro showed that the cell survival rate of the PET-LTPT extract group was 135.82%, higher than that of the negative control group and blank group. It indicated that PET-LTPT material had no cytotoxicity in vitro. The results of the intradermal reaction test showed that the difference between the average score of PET-LTPT in different extraction media and the control group was less than 1.0, and there was no intradermal reaction. The skin sensitization test results showed that the PET-LTPT scaffold material did not produce delayed-type hypersensitivity to guinea pig skin. PET-LTPT is a potential three-dimensional liver tissue culture scaffold material for the bioartificial liver.

Key words: PET, Low temperature plasma, Biosafety evaluation, Artificial liver, Three-dimensional culture scaffold materials