Abstract：The cryopreservation condition of Saussurea medusa callus was studied. The results indicated that preculture, cryoprotectant, pretreatment, recovery and washing conditions affected the survival rate of Saussurea medusa callus. The preculture medium was MS solid culture medium supplemented with 5% dimethyl sulfoxide. The optimum composition of cryoprotectant was 15% ethyleneglycol, 15% simethyl sulfoxide, 30% glycerol and 0.4 mol/L sucrose. The pretreatment temperature and time were 15oC and 10 min, respectively. The recovery temperature was 25~40oC. The survival rate of Saussurea medusa callus was 58.5% after washed by MS liquid medium with 1.2 mol/L sucrose for 3 times (10 min for each time ) at 25oC.