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›› 2002, Vol. 2 ›› Issue (2): 0-0.

• 2 •    

脱臭馏出物中植物甾醇的回收和精制

许文林，王雅琼，鲁萍   

  1. 扬州大学化学化工系，江苏 扬州225002
  • 出版日期:2002-04-20 发布日期:2002-04-20

Plasmid Stability in High Cell Density Cultures of E.coli HB101(pBR322)

YU Guo-ce1, JIAO Rui-shen2, WANG Ji-cheng3, WANG Shu-qing3   

  1. Department of Chemistry and Chemical Engineering, Yangzhou University, Yangzhou, Jiangsu 225002, China
  • Online:2002-04-20 Published:2002-04-20

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摘要： 以脱臭馏出物为原料，采用甲醇酯化、结晶分离、溶剂重结晶净化精制工艺，对回收和精制植物甾醇的过程进行了研究. 实验结果表明，应用该工艺从油脂脱臭馏出物中提取的植物甾醇含量大于95%，过程总收率达80%以上，为从脱臭馏出物中提取植物甾醇的工业化生产提供了技术依据.

关键词: 脱臭馏出物, 植物甾醇, 回收精制

Abstract: The purification and recover processes of phytosterols from deoderizer distillates were studied by the estertification, crystallization and recrystallization technique. The experimental results showed that the purity of photosterols is more than 95% and the total recovery is more than 80%.

Key words: E.coli HB101(pBR322), high cell density culture, plasmid stability, b-lactamase

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