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›› 2013, Vol. 13 ›› Issue (2): 287-291.

• 生化工程专栏 • 上一篇    下一篇

聚(甲基丙烯酸-二乙烯基苯-三烯丙基异三聚氰酸酯)整体柱应用于质粒分析

刘孟儒 刘琳琳 张琪 崔伟佳 张敏莲   

  1. 清华大学化学工程系 北京理工大学化工与环境学院 清华大学化学工程系 清华大学化学工程系 清华大学化学工程系
  • 收稿日期:2013-01-15 修回日期:2013-03-15 出版日期:2013-04-20 发布日期:2013-04-20
  • 通讯作者: 张敏莲

Application of Poly(glycidyl methacrylate-divinylbenzene-triallylisocyanurate) Monolith in Plasmid Analysis

LIU Meng-ru LIU Lin-lin ZHANG Qi CUI Wei-jia ZHANG Min-lian   

  1. Department of Chemical Engineering, Tsinghua University School of Chemical Engineering and the Environment, Beijing Institute of Technology Department of Chemical Engineering, Tsinghua University Department of Chemical Engineering, Tsinghua University Department of Chemical Engineering, Tsinghua University
  • Received:2013-01-15 Revised:2013-03-15 Online:2013-04-20 Published:2013-04-20
  • Contact: ZHANG Min-lian

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摘要： 针对质粒的结构特点，合成了含大量10~300 mm超大孔的阴离子型聚(甲基丙烯酸-二乙烯基苯-三烯丙基异三聚氰酸酯)(PGDT)整体柱，经二乙胺修饰的PGDT具有密度为1.58 mmol/g的阴离子交换功能基团. 将其用于质粒标准品和细胞裂解液的分析，并与市售的无孔微球型色谱柱TSK DNA-NPR和整体柱型色谱柱CIM DEAE Disk Monolithic Column比较. PGDT整体柱分析质粒标准品时能有效将质粒与其他杂质分离，效果与市售TSK和CIM色谱柱相当；分析细胞裂解液时，PGDT整体柱和CIM色谱柱能将RNA、非核酸杂质分离，TSK则仅能将质粒和非质粒分离. 合成的PGDT整体柱对质粒样品有良好的分析能力.

关键词: 聚(甲基丙烯酸-二乙烯基苯-三烯丙基异三聚氰酸酯), 整体柱, 质粒DNA, 分析

Abstract: Anion-exchange poly(glycidyl methacrylate-divinylbenzene-triallylisocyanurate) (PGDT) monolith with superporous pores with the diameters range of 10~300 mm was synthesized, which was used to analyze plasmid standard and cell lysate. The analysis results were compared with commercial columns, including TSK DNA-NPR and CIM DEAE Disk Monolithic Column. The prepared PGDT monolith could effectively absorb plasmid and separate it from impurities, with its results similar to TSK and CIM

Key words: poly(glycidyl methacrylate-divinylbenzene-triallylisocyanurate), monolith, plasmid DNA, analysis

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