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›› 2013, Vol. 13 ›› Issue (2): 302-305.

• 生化工程专栏 • 上一篇    下一篇

Production of Fumaric Acid from Raw Starchy Materials by Rhizopus oryzae with SSF

高敏 徐晓康 徐晴 李霜   

  1. 南京工业大学国家生化中心 南京工业大学国家生化中心 南京工业大学制药与生命科学学院 南京工业大学 制药与生命科学学院
  • 收稿日期:2012-10-18 修回日期:2012-12-17 出版日期:2013-04-20 发布日期:2013-04-20
  • 通讯作者: 高敏

Production of Fumaric Acid from Raw Starchy Materials by Rhizopus oryzae with SSF

GAO Min XU Xiao-kang LI Shuang LI Shuang   

  1. College of Biotechnology and Pharmaceutical Engineering，Nanjing University of Technology College of Biotechnology and Pharmaceutical Engineering，Nanjing University of Technology College of Life Science and Pharmaceutical Engineering. Nanjing University of Technology College of Life Science and Pharmaceutical Engineering. Nanjing University of Technology
  • Received:2012-10-18 Revised:2012-12-17 Online:2013-04-20 Published:2013-04-20
  • Contact: GAO Min

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摘要： To reduce the manufacturing cost of fumaric acid production by fermentation, four kinds of raw starchy materials were investigated, cassava powder, corn powder, degermed corn powder and starch. By comparing the nitrogen contents in them, carbon/nitrogen ratio of raw materials was the key factor affecting the fumaric acid production by Rhizopus oryzae. Based on this point, degermed corn powder was selected as the best raw starchy material for fumaric acid production. In addition, the simultaneous saccharification and fermentation (SSF) process for fumaric acid production was optimized. With degermed corn powder as substrate at 100 g/L total sugar concentration, the fumaric acid production (35.51 g/L), yield (0.355 g/g) and productivity (0.493 [g/(L×h)]) were obtained via SSF process in a 7-L fermentor.

关键词: fumaric acid, starchy, Rhizopus oryzae, carbon/nitrogen ratio, simultaneous saccharification and fermentation

Abstract: To reduce the manufacturing cost of fumaric acid production by fermentation, four kinds of raw starchy materials were investigated, cassava powder, corn powder, degermed corn powder and starch. By comparing the nitrogen contents in them, carbon/nitrogen ratio of raw materials was the key factor affecting the fumaric acid production by Rhizopus oryzae. Based on this point, degermed corn powder was selected as the best raw starchy material for fumaric acid production. In addition, the simultaneous saccharification and fermentation (SSF) process for fumaric acid production was optimized. With degermed corn powder as substrate at 100 g/L total sugar concentration, the fumaric acid production (35.51 g/L), yield (0.355 g/g) and productivity (0.493 [g/(L×h)]) were obtained via SSF process in a 7-L fermentor.

Key words: fumaric acid, starchy, Rhizopus oryzae, carbon/nitrogen ratio, simultaneous saccharification and fermentation

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