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过程工程学报 ›› 2016, Vol. 16 ›› Issue (5): 849-855.DOI: 10.12034/j.issn.1009-606X.216142

• 生化工程专栏 • 上一篇    下一篇

室内外培养对耐温微藻Desmodesmus sp. F51细胞生长和叶黄素积累的影响

谢友坪1,阳需求1,陈剑锋1,卢英华2   

  1. 1. 福州大学生物科学与工程学院
    2. 厦门大学化学工程与生物工程系
  • 收稿日期:2016-02-24 修回日期:2016-04-22 出版日期:2016-10-20 发布日期:2016-10-14
  • 通讯作者: 谢友坪 ypxie@fzu.edu.cn

Effects of Indoor and Outdoor Cultivation Conditions on the Cell Growth and Lutein Accumulation of Thermo-tolerant Desmodesmus sp. F51

XIE You-ping 1,YANG Xu-qiu 1,CHEN Jian-feng 2,LU Ying-hua 3   

  1. 1. College of Biological Science and Engineering, Fuzhou University
    2. College of Biological Science and Technology, Fuzhou University
    3. Department of Chemical and Biochemical Engineering, Xiamen University
  • Received:2016-02-24 Revised:2016-04-22 Online:2016-10-20 Published:2016-10-14
  • Contact: XIE You-ping ypxie@fzu.edu.cn

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被引次数

3

摘要： 在室内培养耐温微藻F51，考察了培养温度、自来水及非灭菌培养基对细胞生长及叶黄素积累的影响，并与50 L柱状光生物反应器户外分批培养情况进行对比. 结果表明，藻株在35℃下的生物质产率和叶黄素产率最大，分别为767.5和3.05 mg/(L?d). 低温培养有利于叶黄素积累，但生长速率减缓. 用自来水及非灭菌培养基培养时，藻株无明显的生长抑制及染菌现象，在13.0?27.6℃户外培养温度下，藻株最高生物质产率、二氧化碳固定率和叶黄素产率分别为236.3, 444.2和1.17 mg/(L?d).

关键词: 耐温, 微藻, 叶黄素, 室内培养, 室外培养

Abstract: The effects of temperature, local tap-water and culture medium without sterilization on cell growth and lutein production of Desmodesmus sp. F51 were investigated under indoor conditions. Outdoor batch cultivation of Desmodesmus sp. F51 was also investigated in 50 L cylindrical photobioreactor. The results showed that the best biomass productivity [767.5 mg/(L?d)] and lutein productivity [3.05 mg/(L?d)] occurred at a temperature of 35℃. A low temperature enhanced the lutein content but reduced cell growth rate. There is no significant growth inhibition and microbiological contamination were observed for Desmodesmus sp. F51 using local tap-water and culture medium without sterilization. Under the outdoor temperature of 13.0?27.6℃, the highest biomass productivity, CO2 fixation rate and lutein productivity of 236.3, 444.2 and 1.17 mg/(L?d) were obtained, respectively.

Key words: thermo-tolerant, microalgae, lutein, indoor cultivation, outdoor cultivation

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