基于离子交换层析技术提取发酵液中的丙酸

doi:10.12034/j.issn.1009-606X.215311

过程工程学报 ›› 2016, Vol. 16 ›› Issue (3): 407-412.DOI: 10.12034/j.issn.1009-606X.215311

基于离子交换层析技术提取发酵液中的丙酸

崔晓蓬杨海朋徐国霞王自强王云山张利平苏志国

河北大学生命科学学院河北大学生命科学学院河北大学生命科学学院中国科学院过程工程研究所生化工程国家重点实验室中国科学院过程工程研究所生化工程国家重点实验室河北大学生命科学学院中国科学院过程工程研究所生化工程国家重点实验室

收稿日期:2015-08-19 修回日期:2016-01-07 出版日期:2016-06-20 发布日期:2017-04-28
通讯作者: 崔晓蓬

Extraction of Propionic Acid from Fermentation Broth by Ion-exchange Chromatography

CUI Xiao-peng YANG Hai-peng XU Guo-xia WANG Zi-qiang WANG Yun-shan， ZHANG Li-ping SU Zhi-guo

(1.College of Life Science, Hebei University (1.College of Life Science, Hebei University (1.College of Life Science, Hebei University Key Lab of Biochemical Engineering, Institute of Process Engineering, State Key Lab. Biochem. Eng., Inst. Process Eng., Chinese Academy of Sciences College of Life Science, Hebei University State Key Lab. Biochem. Eng., Inst. Process Eng., Chinese Academy of Sciences

Received:2015-08-19 Revised:2016-01-07 Online:2016-06-20 Published:2017-04-28
Contact: CUI Xiao-peng

摘要/Abstract

摘要： 用7种不同性能的阴离子交换树脂吸附发酵液中丙酸，考察了发酵液酸化方式及pH值对吸附量的影响，分析了不同浓度H2SO4的解吸情况，比较了多柱串联组合层析提高丙酸收率的可行性. 结果表明，大孔弱碱性丙烯酸系阴离子交换树脂ZGD630对发酵液中的丙酸吸附量最高，采用H型阳离子交换树脂D001酸化工艺，当pH值由6.5降至3时，ZGD630树脂对发酵液中丙酸的静态吸附量从54.34 mg/g提高至110 mg/g，动态吸附量达152.5 mg/g. 用4 mol/L H2SO4进行解吸，丙酸浓度最高达81.8 g/L，为初始发酵液的2.12倍. 采用3柱串联组合层析时丙酸收率达62.15%，较单柱层析显著提高.

关键词: 丙酸丙酸杆菌, 丙酸, 离子交换树脂, 组合层析

Abstract: The adsorption capacity of 7 ion-exchange resins with different properties for propionic acid in the fermentation broth was investigated. The effects of fermentation broth acidification method and pH value on the resin adsorption capacity were studied. The elution processes with different concentrations of H2SO4 were analyzed. The feasibility of in series multi-column chromatography to increase the yield of propionic acid was discussed. The results showed that ZGD630, a macroporous weak-base anion exchange resin had the highest adsorption capacity for propionic acid. D001 H-type cation exchange resin acidization method was determined. When pH value decreased to 3 from 6.5, the static state adsorption capacity of ZGD630 increased from 54.34 mg/g to 110 mg/g dry beads, with a dynamic adsorption capacity of 152.5 mg/g. The highest propionic acid concentration during the elution process (4 mol/L H2SO4) reached 81.8 g/L, which was 2.12 times the original fermentation broth concentration. A recovery yield of 62.15% was obtained for propionic acid with in series three-column combined chromatography, a significant improvement compared to the one-column chromatography.

Key words: Propionibacterium acidipropionici, propionic acid, ion-exchange resin, combined chromatography

中图分类号:

Q939.9

崔晓蓬杨海朋徐国霞王自强王云山张利平苏志国. 基于离子交换层析技术提取发酵液中的丙酸[J]. 过程工程学报, 2016, 16(3): 407-412.

CUI Xiao-peng YANG Hai-peng XU Guo-xia WANG Zi-qiang WANG Yun-shan; ZHANG Li-ping SU Zhi-guo. Extraction of Propionic Acid from Fermentation Broth by Ion-exchange Chromatography[J]. Chin. J. Process Eng., 2016, 16(3): 407-412.

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基于离子交换层析技术提取发酵液中的丙酸

Extraction of Propionic Acid from Fermentation Broth by Ion-exchange Chromatography

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