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过程工程学报 ›› 2016, Vol. 16 ›› Issue (4): 639-646.DOI: 10.12034/j.issn.1009-606X.216134

• 生化工程专栏 • 上一篇    下一篇

壳聚糖微球及其与白油混合乳液对禽用新流二联疫苗佐剂的免疫效果

邹勇娟1,刘艳2,王月琦1,吴颉3,巩方玲4,马光辉4   

  1. 1. 中国科学院大学
    2. 中国科学院过程工程研究所分离工程与工程青年实验室
    3. 国家生化工程技术研究中心
    4. 中国科学院过程工程研究所生化工程国家重点实验室
  • 收稿日期:2016-01-29 修回日期:2016-03-23 出版日期:2016-08-20 发布日期:2016-10-10
  • 通讯作者: 邹勇娟 yjzou@ipe.ac.cn

Immune Effect of HTCC Hydrogel Microspheres and the Blending Emulsion of Oil and Microspheres as New Adjuvants of Combined Vaccine against Newcastle Disease and Avian Influenza

ZOU Yong-juan 1,LIU Yan 2,WANG Yue-qi 1,WU Jie 3,GONG Fang-ling, 4,MA Guang-hui 5   

  1. 1. University of Chinese Academy of Sciences
    2. Lab. Sep. Sci. & Eng., Inst. Process Eng., CAS
    3. National Engineering Research Center for Biotechnology
    4. State Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences
    5. State Key Lab. Biochem. Eng., Inst. Process Eng., Chinese Academy of Sciences
  • Received:2016-01-29 Revised:2016-03-23 Online:2016-08-20 Published:2016-10-10
  • Contact: ZOU Yong-juan yjzou@ipe.ac.cn

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摘要： 用不同粒径的壳聚糖季铵盐凝胶微球及其与白油的混合乳液吸附新城疫及禽流感灭活抗原，评价其对新流二联灭活疫苗的免疫增强效果. 结果表明，吸附时间2 h、微球浓度2 mg/mL、微球粒径1 ?m为吸附新流二联灭活抗原的最佳条件，抗原吸附率达90%. 免疫SPF鸡后，壳聚糖季铵盐微球及混合乳液均能显著提高新城疫及禽流感病毒特异性抗体效价，且混合乳液组显著优于微球组. 与白油佐剂相比，壳聚糖季铵盐微球及混合乳液均能显著增强细胞免疫，淋巴细胞增殖水平为纯抗原组的1.5倍，具有用作禽类疫苗佐剂的巨大潜力.

关键词: 壳聚糖季铵盐凝胶微球, 混合乳液, 新流二联疫苗, 佐剂, 细胞免疫

Abstract: Aiming to enhance the immune efficacy of the combined vaccine against newcastle disease and avian influenza, N-[(2-hydroxy-3-trimethylammonium) propyl] chitosan chloride (HTCC) hydrogel microspheres and the blending emulsion of oil with microspheres (MPs) were investigated as vaccine adjuvants. After optimization, the maximized antigen absorption efficiency reached 90% when the antigens were incubated with 2 mg/mL microspheres with the size of 1 ?m for 2 h. In vivo experiments indicated that both the microspheres and blending emulsion significantly enhance the antigen specific HI titers. In detail, the blending emulsion group was superior to the microspheres in the enhancement of HI titers. Most importantly, both the microspheres and blending emulsion could induce robust and long-term cellular immune responses compared to oil adjuvant, and the proliferation levels of peripheral blood mononuclear cells were 1.5 folds to that of the soluble antigen group. These results suggested that the HTCC hydrogel microspheres and blending emulsion would be promising adjuvants for poultry vaccine.

Key words: N-[(2-hydroxy-3-trimethylammonium) propyl] chitosan chloride, blending emulsion, combined vaccine against newcastle disease and avian influenza, adjuvant, cellular immunity

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