赤霉素发酵过程动力学建模与分析

doi:10.12034/j.issn.1009-606X.216304

过程工程学报 ›› 2017, Vol. 17 ›› Issue (3): 605-612.DOI: 10.12034/j.issn.1009-606X.216304

赤霉素发酵过程动力学建模与分析

王卫^*，吴耀辉，黎继烈，姚跃飞

中南林业科技大学生命科学与技术学院，湖南长沙 410004

收稿日期:2016-09-22 修回日期:2016-11-23 出版日期:2017-06-20 发布日期:2017-06-14
通讯作者: 王卫 wanderwish@126.com
基金资助:
湖南省教育厅一般资助项目

Construction and Analysis on the Kinetic Model of Gibberellin Acid in Batch Fermentation

Wei WANG^*, Yaohui WU, Jilie LI, Yuefei YAO

College of Life Science and Technology, Central South University of Forestry and Technology, Changsha, Hunan 410004, China

Received:2016-09-22 Revised:2016-11-23 Online:2017-06-20 Published:2017-06-14
Contact: Wei WANG wanderwish@126.com

摘要/Abstract

摘要：

采用5 L全自动发酵罐对赤霉菌进行发酵培养，分时取样，记录不同时段发酵液中菌丝浓度、残糖浓度、氨基氮浓度及赤霉素浓度；将赤霉素发酵过程分为菌丝营养生长阶段(0~64 h)与菌丝产赤霉素阶段(64~192 h)，分段建立发酵动力学模型，对赤霉素发酵培养基中初始碳源、氮源浓度及碳氮质量比(C/N)进行优化. 结果表明，在考察的碳源、氮源浓度适用范围内，模型计算值与实验值吻合度较好.

关键词: 赤霉素, 赤霉菌, 分批发酵, 动力学模型

Abstract:

The concentration changes of mycelium, residual sugar, amino nitrogen and gibberellic acid (GA₃) were recorded in a 5 L automatic fermenter. Based on the analysis of fermentation process, a two-stage differential kinetic model, including mycelium growth stage (0~64 h) and GA₃ production phase (64~192 h), was constructed. By using the model, the initial carbon, nitrogen concentration and carbon-nitrogen mass ratio (C/N) in the fermentation medium were optimized. Within the scope of the carbon concentration and nitrogen concentration, the calculated results of models were compared satisfactorily with experimental data.

Key words: gibberellic acid, Gibberella fujikuroi, batch fermentation, kinetic model

王卫吴耀辉黎继烈姚跃飞. 赤霉素发酵过程动力学建模与分析[J]. 过程工程学报, 2017, 17(3): 605-612.

Wei WANG Yaohui WU Jilie LI Yuefei YAO. Construction and Analysis on the Kinetic Model of Gibberellin Acid in Batch Fermentation[J]. Chin. J. Process Eng., 2017, 17(3): 605-612.

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赤霉素发酵过程动力学建模与分析

Construction and Analysis on the Kinetic Model of Gibberellin Acid in Batch Fermentation

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