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过程工程学报 ›› 2017, Vol. 17 ›› Issue (3): 506-511.DOI: 10.12034/j.issn.1009-606X.217174

• 反应与分离 • 上一篇    下一篇

牛初乳中唾液酸乳糖和乳糖的色谱分离

李明1,周家春1,秦臻1,陈启明1,邱勇隽1,赵黎明2   

  1. 华东理工大学生物工程学院，发酵工业分离提取技术研发中心，生物反应器工程国家重点实验室，上海 200237
  • 收稿日期:2017-03-13 修回日期:2017-04-13 出版日期:2017-06-20 发布日期:2017-06-14
  • 通讯作者: 赵黎明 zhaoliming@ecust.edu.cn

Chromatographic Separation of Sialyllactose and Lactose in Bovine Coloctrum

Ming LI,   Jiachun ZHOU*,   Zhen QIN,   Qiming CHEN,   Yongjun QIU,   Liming ZHAO*   

  1. State Key Laboratory of Bioreactor Engineering, R&D Center of Separation and Extraction Technology in Fermentation Industry, School of Biotechnology, East China University of Science and Technology, Shanghai 200237, China
  • Received:2017-03-13 Revised:2017-04-13 Online:2017-06-20 Published:2017-06-14
  • Contact: Li-ming ZHAO zhaoliming@ecust.edu.cn

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摘要：

采用纳滤及一价选择性离子交换膜电渗析2种方法对模拟浓海水进行分离浓缩，探索一、二价离子(Na+/Mg2+, Cl-/SO42-)的分离效果. 实验表明，DL2540膜对二价离子的截留性能优于NF270膜，在2.1 MPa压力、浓侧流量为300 L/h的条件下，对Mg2+和SO42-的截留率分别达98.1%和80.5%，分离因子为2.5和40.0. 一价离子选择性电渗析膜对阴离子Cl-和SO42-的分离效果明显好于阳离子Na+和Mg2+，Na+回收率为90%时，选择性迁移比分别为18.4和2.9. 纳滤和选择性电渗析对一、二价离子都有较好的分离效果，电渗析离子分离的同时可实现浓缩，而纳滤对一价离子几乎无浓缩效果.

关键词: 唾液酸乳糖, 乳糖, 色谱分离, 牛初乳

Abstract:

Electrodialysis (ED) and nanofiltration (NF) were adopted to separate monovalent and divalent ions (Na+/Mg2+, Cl-/SO42-). The results showed that DL2540 had a better separation efficiency for the rejection of divalent ions than NF270. The rejection of Mg2+ and SO42- were 98.1% and 80.5%, respectively. For ED, the separation factors were 2.5 and 40.0. The separation performance of Cl-/SO42- was better than that of Na+/Mg2+, the separation factors were 18.4 and 2.9 under 90% recover rate of Na+. Both methods had good separation effects of monovalent and divalent ions. ED could also realize ions concentration, while NF could not.

Key words: sialyllactose, lactose, chromatographic separate, bovine coloctrum